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Literature summary extracted from

  • Zhang, B.; Li, P.; Zhang, H.; Fan, L.; Wang, H.; Li, X.; Tian, L.; Ali, N.; Ali, Z.; Zhang, Q.
    Papain/Zn3(PO4)2 hybrid nanoflower Preparation, characterization and its enhanced catalytic activity as an immobilized enzyme (2016), RSC Adv., 6, 46702-46710 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.22.2 industry catalytic properties of papain immobilized on hybrid nanoflowers are enhanced compared with that of free papain. The hybrid nanoflowers exhibit excellent reusability, high thermostability, long storage life and great potential in industrial applications Carica papaya

General Stability

EC Number General Stability Organism
3.4.22.2 papain immobilized on hybrid nanoflowers retains 88.8% of its initial activity after ten successive cycles of reuse Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 additional information
-
casein 0.245 g/ml (soluble enzyme), 0.252 g/ml (papain immobilized on hybrid nanoflowers), pH 7.4, 37°C Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya P00784
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 casein + H2O
-
Carica papaya ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 55
-
soluble enzyme Carica papaya
3.4.22.2 70
-
papain immobilized on hybrid nanoflowers Carica papaya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.2 25 70 25°C: about 65% of maximal activity, 70°C: about 60% of maximal activity, soluble enzyme Carica papaya
3.4.22.2 25 90 25°C: about 50% of maximal activity, 90°C: about 70% of maximal activity, papain immobilized on hybrid nanoflowers Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.2 55
-
390 min, the soluble enzyme retains 41.9% of its activity, enzyme immobilized on hybrid nanoflowers retains 77.2% of its activity Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6.8
-
soluble enzyme Carica papaya
3.4.22.2 7.2
-
papain immobilized on hybrid nanoflowers Carica papaya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.2 6.4 9.4 pH 6.4: about 85% of maximal activity, pH 9.4: about 50% of maximal activity Carica papaya
3.4.22.2 6.4 10.2 pH 6.4: about 85% of maximal activity, pH 10.2: about 70% of maximal activity Carica papaya