EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.22.2 | industry | catalytic properties of papain immobilized on hybrid nanoflowers are enhanced compared with that of free papain. The hybrid nanoflowers exhibit excellent reusability, high thermostability, long storage life and great potential in industrial applications | Carica papaya |
EC Number | General Stability | Organism |
---|---|---|
3.4.22.2 | papain immobilized on hybrid nanoflowers retains 88.8% of its initial activity after ten successive cycles of reuse | Carica papaya |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.2 | additional information | - |
casein | 0.245 g/ml (soluble enzyme), 0.252 g/ml (papain immobilized on hybrid nanoflowers), pH 7.4, 37°C | Carica papaya |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.2 | Carica papaya | P00784 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.2 | casein + H2O | - |
Carica papaya | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 55 | - |
soluble enzyme | Carica papaya |
3.4.22.2 | 70 | - |
papain immobilized on hybrid nanoflowers | Carica papaya |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 25 | 70 | 25°C: about 65% of maximal activity, 70°C: about 60% of maximal activity, soluble enzyme | Carica papaya |
3.4.22.2 | 25 | 90 | 25°C: about 50% of maximal activity, 90°C: about 70% of maximal activity, papain immobilized on hybrid nanoflowers | Carica papaya |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 55 | - |
390 min, the soluble enzyme retains 41.9% of its activity, enzyme immobilized on hybrid nanoflowers retains 77.2% of its activity | Carica papaya |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 6.8 | - |
soluble enzyme | Carica papaya |
3.4.22.2 | 7.2 | - |
papain immobilized on hybrid nanoflowers | Carica papaya |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 6.4 | 9.4 | pH 6.4: about 85% of maximal activity, pH 9.4: about 50% of maximal activity | Carica papaya |
3.4.22.2 | 6.4 | 10.2 | pH 6.4: about 85% of maximal activity, pH 10.2: about 70% of maximal activity | Carica papaya |