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Literature summary extracted from

  • Wan, H.; Li, D.
    Highly efficient biotransformation of ginsenoside Rb1 and Rg3 using beta-galactosidase from Aspergillus sp. (2015), RSC Adv., 5, 78874-78879 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.191 synthesis the beta-galactosidase from Aspergillus sp. can be useful for the mass production of rare ginsenosides Aspergillus sp.
3.2.1.192 synthesis the beta-galactosidase from Aspergillus sp. can be useful for the mass production of rare ginsenosides Aspergillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.191 Aspergillus sp.
-
-
-
3.2.1.192 Aspergillus sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.191 0.014
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.
3.2.1.191 6.54
-
 substrate 2-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.
3.2.1.192 0.014
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.
3.2.1.192 6.54
-
 substrate 2-nitrophenyl beta-D-glucopyranoside, pH 5.6, 37°C Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.191 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 ginsenoside Rb1 + H2O cf. EC 3.2.1.192 Aspergillus sp. ginsenoside Rd + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rd + H2O
-
 Aspergillus sp. ginsenoside F2 + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rg3 + H2O
-
 Aspergillus sp. ginsenoside Rh2 + D-glucopyranose
-
 ?
3.2.1.191 additional information beta-galactosidase from Aspergillus sp. displays beta-glucosidase activity, which is responsible for its ability to transform major ginsenoside Rb1 to rare ginsenoside F2 and to ginsenoside Rg3 via ginsenoside Rd. Ginsenoside Rg3 can be selectively hydrolyzed and only Rh2 is obtained with this beta-galactosidase. Before hydrolysis, an Rg3 inclusion complex is prepared with hydroxypropyl-b-cyclodextrin (HP-beta-CD) to improve the aqueous solubility. The solubility of Rg3 increases 74.6fold, and the phase solubility curve displays a typical AL-type, which indicates the formation of a 1:1 inclusion complex. Using an enzyme loading of 500 U/g Rg3, the highest Rg3 conversion of 90.6% and Rh2 yield of 88.5% are obtained after 24 h at 60°C. Ginsenoside F2 is not converted into ginsenoside Rh2 and this is not converted into the protopanaxadiol aglycone Aspergillus sp. ?
-
 ?
3.2.1.192 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.192 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.192 ginsenoside Rb1 + H2O
-
 Aspergillus sp. ginsenoside Rd + D-glucopyranose
-
 ?
3.2.1.192 ginsenoside Rd + H2O
-
 Aspergillus sp. ginsenoside Rg3 + D-glucopyranose
-
 ?
3.2.1.192 additional information beta-galactosidase from Aspergillus sp. displays beta-glucosidase activity, which is responsible for its ability to transform major ginsenoside Rb1 to rare ginsenoside F2 and to ginsenoside Rg3 via ginsenoside Rd. Ginsenoside Rg3 can be selectively hydrolyzed and only Rh2 is obtained with this beta-galactosidase. Before hydrolysis, an Rg3 inclusion complex is prepared with hydroxypropyl-b-cyclodextrin (HP-beta-CD) to improve the aqueous solubility. The solubility of Rg3 increases 74.6fold, and the phase solubility curve displays a typical AL-type, which indicates the formation of a 1:1 inclusion complex. Using an enzyme loading of 500 U/g Rg3, the highest Rg3 conversion of 90.6% and Rh2 yield of 88.5% are obtained after 24 h at 60°C. Ginsenoside F2 is not converted into ginsenoside Rh2 and this is not converted into the protopanaxadiol aglycone Aspergillus sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.191 More cf. EC 3.2.1.192 Aspergillus sp.
3.2.1.192 More cf. EC 3.2.1.191 Aspergillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.191 37
-
 assay at Aspergillus sp.
3.2.1.192 37
-
 assay at Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.191 5.6
-
 assay at Aspergillus sp.
3.2.1.192 5.6
-
 assay at Aspergillus sp.