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Literature summary extracted from

  • Mangels, N.; Awwad, K.; Wettenmann, A.; Dos Santos, L.R.; Froemel, T.; Fleming, I.
    The soluble epoxide hydrolase determines cholesterol homeostasis by regulating AMPK and SREBP activity (2016), Prostaglandins Other Lipid Mediat., 125, 30-39 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.10 gene EPHX2, quantitative real-time PCR expression analysis Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.10 additional information construction of sEH-/- knockout mice, phenotype compared to wild-type, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 trans-4-[4-(3-adamantan-1-ylureido)cyclohexyloxy]-benzoic acid i.e. t-AUCB Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 cytosol
-
 Mus musculus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Mus musculus P34914
-
-
3.3.2.10 Mus musculus C57BL/6 P34914
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.10 hepatocyte
-
 Mus musculus
-
3.3.2.10 kidney
-
 Mus musculus
-
3.3.2.10 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (9Z)-12,13-epoxyoctadecenoic acid + H2O
-
 Mus musculus (9Z)-12,13-dihydroxyoctadecenoic acid
-
 ?
3.3.2.10 (9Z)-12,13-epoxyoctadecenoic acid + H2O
-
 Mus musculus C57BL/6 (9Z)-12,13-dihydroxyoctadecenoic acid
-
 ?
3.3.2.10 additional information the sEH is a dual function enzyme that generates dihydroxy-fatty acids via its C-terminal hydrolase domain, while the N-terminal phosphatase domain has been proposed to have lipid phosphatase activity, bifunctional epoxide hydrolase 2 includes cytosolic epoxide hydrolase 2, sEH, EC 3.3.2.10, and lipid-phosphate phosphatase, EC 3.1.3.76 Mus musculus ?
-
 ?
3.3.2.10 additional information the sEH is a dual function enzyme that generates dihydroxy-fatty acids via its C-terminal hydrolase domain, while the N-terminal phosphatase domain has been proposed to have lipid phosphatase activity, bifunctional epoxide hydrolase 2 includes cytosolic epoxide hydrolase 2, sEH, EC 3.3.2.10, and lipid-phosphate phosphatase, EC 3.1.3.76 Mus musculus C57BL/6 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.10 SEH
-
 Mus musculus

General Information

EC Number General Information Comment Organism
3.3.2.10 malfunction deletion of sEH decreases expression of HMG-CoA reductase, fatty acid synthase, and low density lipoprotein receptor. Sterol regulatory element binding proteins (SREBPs) regulate the expression of all three enzymes and SREBP activation is attenuated in the absence of sEH. The effect is attributed to the AMPK-activated protein kinase (AMPK) which is activated in the absence of sEH. Livers from wild-type versus sEH-/- littermates contain significantly higher levels of the sEH substrate 12,13-epoxyoctadecenoic acid, which elicits dAMPK activation, while the corresponding sEH product is inactive. Thus, AMPK activation and subsequent inhibition of SREBP can account for the altered expression of lipid metabolizing enzymes in sEH-/- mice Mus musculus