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Literature summary extracted from

  • McCabe, M.T.; Graves, A.P.; Ganji, G.; Diaz, E.; Halsey, W.S.; Jiang, Y.; Smitheman, K.N.; Ott, H.M.; Pappalardi, M.B.; Allen, K.E.; Chen, S.B.; Della Pietra, A.; Dul, E.; Hughes, A.M.; Gilbert, S.A.; Thrall, S.H.; Tummino, P.J.; Kruger, R.G.; Brandt, M.; Schwartz, B.; Creasy, C.L.
    Mutation of A677 in histone methyltransferase EZH2 in human B-cell lymphoma promotes hypertrimethylation of histone H3 on lysine 27 (H3K27) (2012), Proc. Natl. Acad. Sci. USA, 109, 2989-2994 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.356 A677G the mutant cell line reveals aberrantly elevated trimethylated [histone H3]-L-lysine27 and decreased monomethylated and dimethylated [histone H3]-L-lysine27 as compared to the wild type. The mutant enzyme demonstrates nearly equal efficiency for all three substrates (unmethylated H3K27:monomethylated H3K27:dimethylated H3K27 kcat/Km ratio is 1.1:0.6:1) Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.356 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Homo sapiens highest activity S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.356 Homo sapiens Q15910
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.356 B-cell lymphoma cell
-
 Homo sapiens
-
2.1.1.356 Pfeiffer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.356 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 additional information the enzyme displays a preference for substrates with less methylation (unmethylated H3K27:monomethylated H3K27:dimethylated H3K27 kcat/Km ratio is 9:6:1) Homo sapiens ?
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 highest activity Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.356 EZH2 component of the polycomb repressive complex 2 Homo sapiens