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Literature summary extracted from

  • Devaraj, Y.; Rajender, S.K.; Halami, P.M.
    Purification and characterization of fibrinolytic protease from Bacillus amyloliquefaciens MCC2606 and analysis of fibrin degradation product by MS/MS (2018), Prep. Biochem. Biotechnol., 48, 172-180 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 2-mercaptoethanol 95.08% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Al3+ 3.5% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Ba2+ 79.86% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Ca2+ 87.04% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Cs2+ 89.14% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Cu2+ 4.55% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 dithiothreitol 84.92% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 EDTA 2.15% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 iodoacetic acid 80.54% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 K+ 86.69% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Mg2+ 92.64% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 Na+ 84.41% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 pepstatin A 84.31% residual activity at 10 mM Bacillus amyloliquefaciens
3.4.24.72 phenylmethylsulfonyl fluoride 8.62% residual activity at 10 mM Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Mn2+ the activity of the enzyme is enhanced to 111.21% in the presence of 10 mM Mn2+ Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Bacillus amyloliquefaciens
-
-
-
3.4.24.72 Bacillus amyloliquefaciens MCC2606
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 ammonium sulfate precipitation, DEAE-cellulose ion-exchange column chromatography and Sephadex G-75 gel filtration Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 Bovine serum albumin + H2O 16.62% activity compared to human fibrin Bacillus amyloliquefaciens ?
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 ?
3.4.24.72 Bovine serum albumin + H2O 16.62% activity compared to human fibrin Bacillus amyloliquefaciens MCC2606 ?
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 ?
3.4.24.72 casein + H2O 60.36% activity compared to human fibrin Bacillus amyloliquefaciens ?
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 ?
3.4.24.72 casein + H2O 60.36% activity compared to human fibrin Bacillus amyloliquefaciens MCC2606 ?
-
 ?
3.4.24.72 fibrinogen + H2O 55.21% activity compared to human fibrin. Among the three chains of fibrinogen, Aalpha and Bbeta chains are degraded rapidly, whereas gamma-chain of fibrinogen shows significant resistance to degradation by the enzyme Bacillus amyloliquefaciens fibrin + ?
-
 ?
3.4.24.72 fibrinogen + H2O 55.21% activity compared to human fibrin. Among the three chains of fibrinogen, Aalpha and Bbeta chains are degraded rapidly, whereas gamma-chain of fibrinogen shows significant resistance to degradation by the enzyme Bacillus amyloliquefaciens MCC2606 fibrin + ?
-
 ?
3.4.24.72 Hemoglobin + H2O 25.09% activity compared to human fibrin Bacillus amyloliquefaciens ?
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 ?
3.4.24.72 Hemoglobin + H2O 25.09% activity compared to human fibrin Bacillus amyloliquefaciens MCC2606 ?
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 ?
3.4.24.72 human fibrin + H2O fibrin is the most preferred substrate (100% activity). The beta-chain of fibrin is the primary component and site susceptible for the enzyme in early action. The enzyme can completely degrade all the four chains of fibrin Bacillus amyloliquefaciens ?
-
 ?
3.4.24.72 human fibrin + H2O fibrin is the most preferred substrate (100% activity). The beta-chain of fibrin is the primary component and site susceptible for the enzyme in early action. The enzyme can completely degrade all the four chains of fibrin Bacillus amyloliquefaciens MCC2606 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 ? x * 32000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 CFR15-protease
-
 Bacillus amyloliquefaciens
3.4.24.72 fibrinolytic protease
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 45
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.72 30 60 the enzyme is active between 30 and 60°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 10.5
-
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.72 5.5 11 the enzyme is active between pH 5.5 and 11.0 Bacillus amyloliquefaciens