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Literature summary extracted from

  • Zapata-Perez, R.; Martinez-Monino, A.B.; Garcia-Saura, A.G.; Cabanes, J.; Takami, H.; Sanchez-Ferrer, A.
    Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community (2017), PLoS ONE, 12, e0181561 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 wild type enzyme and four mutants enzymes are produced in Escherichia coli Rosetta 2 (DE3)pLysS uncultured Acidobacteria bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 5-bromonicotinaldehyde
-
 uncultured Acidobacteria bacterium
3.5.1.19 nicotinaldehyde
-
 uncultured Acidobacteria bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.1
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 0.12
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 0.14
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.15
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.21
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 0.21
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 0.22
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 0.23
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.23
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.33
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 0.39
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 0.41
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.46
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.59
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.67
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.68
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.74
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 0.89
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 1.08
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 1.09
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 1.21
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 1.31
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 2.89
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 3.59
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 3.84
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Fe2+ the enzyme contains a Fe2+ ion in its metal binding site, which is coordinated by an aspartate (D67) and three histidine residues (H69, H75 and H86) uncultured Acidobacteria bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 21200
-
 gel filtration uncultured Acidobacteria bacterium
3.5.1.19 23900
-
 HPLC/ESI-MS uncultured Acidobacteria bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 uncultured Acidobacteria bacterium H5SPS2 nicotinamidase obtained from a metagenomic library fosmid clone (JFF054_F02) obtained from a geothermal water stream microbial mat community in a Japanese epithermal mine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19
-
 uncultured Acidobacteria bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 5-methylnicotinamide + H2O
-
 uncultured Acidobacteria bacterium 5-methylnicotinate + NH3
-
 ?
3.5.1.19 ethylnicotinate + H2O
-
 uncultured Acidobacteria bacterium nicotinate + ethanol
-
 ?
3.5.1.19 methylnicotinate + H2O
-
 uncultured Acidobacteria bacterium nicotinate + methanol
-
 ?
3.5.1.19 nicotinamide + H2O
-
 uncultured Acidobacteria bacterium nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 uncultured Acidobacteria bacterium pyrazinoic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.19 ? x * 21200, HPLC/ESI-MS uncultured Acidobacteria bacterium

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 UbNic
-
 uncultured Acidobacteria bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.19 90
-
 at pH 7.3 and pH 9.5 uncultured Acidobacteria bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.19 40 100 40°C: about 50% of maximal activity, 100°C: about 70% of maximal activity uncultured Acidobacteria bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.19 additional information
-
 ammonium sulfate at 1 M is able to produce a slight increase in its Tm (86.4°C) in comparison with that found in sodium phosphate pH 7.3. The nicotinamidase reaction products, nicotinic and pyrazinoic acids improved UbNic stability, increasing its Tm to 87.7°C and 87.1°C. Nicotinaldehyde and 5-bromonicotinaldehyde, increased Tm up to 92.7°C and 85.7°C uncultured Acidobacteria bacterium
3.5.1.19 71.3
-
 melting temperature in glycine (pH 10.0) uncultured Acidobacteria bacterium
3.5.1.19 73.1
-
 melting temperature in CAPS (pH 11.0) uncultured Acidobacteria bacterium
3.5.1.19 73.1
-
 melting temperature in sodium acetate (pH 4.0) uncultured Acidobacteria bacterium
3.5.1.19 84.7
-
 melting temperature in glycine buffer pH 9.0 uncultured Acidobacteria bacterium
3.5.1.19 84.8
-
 melting temperature in sodium phosphate buffer pH 7.3 uncultured Acidobacteria bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.19 0.1
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 0.3
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 1.3
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 1.9
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 3.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 3.5
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 3.7
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 4.3
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 4.9
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 4.9
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 5.1
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 5.8
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 7.5
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 9.6
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 10.2
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 12.4
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 12.5
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 13.7
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 15.5
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 18.7
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 22.7
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 43.7
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 54.5
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 9.5 10
-
 uncultured Acidobacteria bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.19 6 11 pH 6.0: about 60% of maximal activity, pH 11.0: about 75% of maximal activity uncultured Acidobacteria bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.19 5 9.5 very stable nicotinamidase from acid (pH 5.0) to basic pHs (up to pH 9.5). Its stability is higher in sodium phosphate pH 7.3 and in glycine pH 9.5, maintaining 90% activity after 24 hours of incubation at such pHs. At lower pH values, the enzyme is less stable. It maintains 75% activity in sodium acetate pH 5.0 and pH 6.0 after 24 hours uncultured Acidobacteria bacterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.19 0.00018
-
 nicotinaldehyde 37°C, pH 7.3 uncultured Acidobacteria bacterium
3.5.1.19 0.00147
-
 5-bromonicotinaldehyde 37°C, pH 7.3 uncultured Acidobacteria bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.19 0.1
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 0.3
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 1.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 1.4
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 2.2
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 2.8
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 2.8
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 5.2
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 14.1
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 18.5
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 20.5
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 34.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 35
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 39.7
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5.1.19 40.1
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 45.7
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 50.5
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 51
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
3.5.1.19 53.9
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 54.3
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.5.1.19 56.7
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
3.5.1.19 68
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.5.1.19 189.2
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium