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Literature summary extracted from

  • Stressler, T.; Ewert, J.; Merz, M.; Funk, J.; Claassen, W.; Lutz-Wahl, S.; Schmidt, H.; Kuhn, A.; Fischer, L.
    A novel glutamyl (aspartyl)-specific aminopeptidase A from Lactobacillus delbrueckii with promising properties for application (2016), PLoS ONE, 11, e0152139 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.7 E64 slight activation Lactobacillus delbrueckii subsp. lactis
3.4.11.21 E64 slight activation Lactobacillus delbrueckii subsp. lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.7 gene pepA, recombinant expression of C-terminally His6-tagged enzyme and of untagged enzyme in Escherichia coli strain BL21(DE3) Lactobacillus delbrueckii subsp. lactis
3.4.11.7 gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Lactococcus lactis ssp. lactis
3.4.11.21 gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Lactococcus lactis ssp. lactis
3.4.11.21 gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Lactobacillus delbrueckii subsp. lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.7 1,10-phenanthroline 21% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 1,10-phenanthroline complete inhibition at 1-10 mM Lactococcus lactis ssp. lactis
3.4.11.7 2-mercaptoethanol 58% inhibition at 0.01 mM, 96% at 0.1 mM Lactococcus lactis ssp. lactis
3.4.11.7 acetone 21.5% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.7 acetone 37.2% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.7 dimethylformamide 78.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.7 dimethylformamide 84.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.7 DMSO 15.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.7 DMSO 13.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.7 DTT 12% inhibition at 0.1 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 DTT 69% inhibition at 0.001 mM, 94% at 0.1 mM Lactococcus lactis ssp. lactis
3.4.11.7 E64 slight inhibition Lactococcus lactis ssp. lactis
3.4.11.7 EDTA complete inhibition at 1.0 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 EDTA complete inhibition at 1.0 mM Lactococcus lactis ssp. lactis
3.4.11.7 ethanol 16.3% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.7 ethanol 25.7% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.7 imidazole 34% inhibition at 40 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 imidazole 21% inhibition at 40 mM Lactococcus lactis ssp. lactis
3.4.11.7 L-Asp 39.5% inhibition at 10 mM, no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 33.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactobacillus delbrueckii subsp. lactis
3.4.11.7 L-Asp 21.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 24.0% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
3.4.11.7 L-Glu product inhibition of enzyme Lc-PepA, 8.4% inhibition at 0.1 mM, 17.7% at 10 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 1.47% inhibition at 0.1 mM, 31.4% at 10 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
3.4.11.7 additional information enzyme Lb-PepA is not product-inhibited by L-Glu. Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined. Poor inhibition at 1.0 mM 2-mercaptoethanol, no inhibition by pepstatin A at 0.1-10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 pepstatin A slight inhibition Lactococcus lactis ssp. lactis
3.4.11.7 PMSF 35% inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 PMSF 32% inhibition at 10 mM Lactococcus lactis ssp. lactis
3.4.11.7 SDS 73% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 SDS 63% at 1.0 mM, 97% inhibition at 10 mM Lactococcus lactis ssp. lactis
3.4.11.21 1,10-phenanthroline 21% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 1,10-phenanthroline complete inhibition at 1-10 mM Lactococcus lactis ssp. lactis
3.4.11.21 2-mercaptoethanol 58% inhibition at 0.01 mM, 96% at 0.1 mM Lactococcus lactis ssp. lactis
3.4.11.21 acetone 21.5% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.21 acetone 37.2% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.21 dimethylformamide 78.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.21 dimethylformamide 84.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.21 DMSO 15.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.21 DMSO 13.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.21 DTT 12% inhibition at 0.1 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 DTT 69% inhibition at 0.001 mM, 94% at 0.1 mM Lactococcus lactis ssp. lactis
3.4.11.21 E64 slight inhibition Lactococcus lactis ssp. lactis
3.4.11.21 EDTA complete inhibition at 1.0 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 EDTA complete inhibition at 1.0 mM Lactococcus lactis ssp. lactis
3.4.11.21 ethanol 16.3% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
3.4.11.21 ethanol 25.7% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
3.4.11.21 Imidazol 34% inhibition at 40 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 Imidazol 21% inhibition at 40 mM Lactococcus lactis ssp. lactis
3.4.11.21 L-Asp 39.5% inhibition at 10 mM, no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 33.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactobacillus delbrueckii subsp. lactis
3.4.11.21 L-Asp 21.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 24.0% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
3.4.11.21 L-Glu product inhibition of enzyme Lc-PepA, 8.4% inhibition at 0.1 mM, 17.7% at 10 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 1.47% inhibition at 0.1 mM, 31.4% at 10 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
3.4.11.21 additional information enzyme Lb-PepA is not product-inhibited by L-Glu. Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined. Poor inhibition at 1.0 mM 2-mercaptoethanol, no inhibition by pepstatin A at 0.1-10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 pepstatin A slight inhibition Lactococcus lactis ssp. lactis
3.4.11.21 PMSF 35% inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 PMSF 32% inhibition at 10 mM Lactococcus lactis ssp. lactis
3.4.11.21 SDS 73% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 SDS 63% at 1.0 mM, 97% inhibition at 10 mM Lactococcus lactis ssp. lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.7 additional information
-
 additional information Michaelis-Menten kinetics Lactococcus lactis ssp. lactis
3.4.11.7 additional information
-
 additional information Michaelis-Menten kinetics Lactobacillus delbrueckii subsp. lactis
3.4.11.7 0.19
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
3.4.11.7 0.34
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
3.4.11.7 1.21
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis
3.4.11.7 25.2
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis
3.4.11.21 additional information
-
 additional information Michaelis-Menten kinetics Lactococcus lactis ssp. lactis
3.4.11.21 additional information
-
 additional information Michaelis-Menten kinetics Lactobacillus delbrueckii subsp. lactis
3.4.11.21 0.19
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
3.4.11.21 0.34
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
3.4.11.21 1.21
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis
3.4.11.21 25.2
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.7 Co2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
3.4.11.7 Co2+ activates, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 Mn2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
3.4.11.7 Mn2+ activates, best at 1.25 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.7 additional information Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined Lactobacillus delbrueckii subsp. lactis
3.4.11.7 additional information Lc-PepA activity decreases only slightly or is almost constant with metal salt concentrations above the optimum concentrations determined Lactococcus lactis ssp. lactis
3.4.11.7 Zn2+ activates, best at 5 mM Lactococcus lactis ssp. lactis
3.4.11.7 Zn2+ slight activation, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 Co2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
3.4.11.21 Co2+ activates, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 Mn2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
3.4.11.21 Mn2+ activates, best at 1.25 mM Lactobacillus delbrueckii subsp. lactis
3.4.11.21 additional information Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined Lactobacillus delbrueckii subsp. lactis
3.4.11.21 additional information Lc-PepA activity decreases only slightly or is almost constant with metal salt concentrations above the optimum concentrations determined Lactococcus lactis ssp. lactis
3.4.11.21 Zn2+ activates, best at 5 mM Lactococcus lactis ssp. lactis
3.4.11.21 Zn2+ slight activation, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.7 470000
-
 recombinant His6-tagged enzyme, gel filtration Lactococcus lactis ssp. lactis
3.4.11.7 508000
-
 recombinant His6-tagged enzyme, gel filtration Lactobacillus delbrueckii subsp. lactis
3.4.11.21 470000
-
 recombinant His6-tagged enzyme, gel filtration Lactococcus lactis ssp. lactis
3.4.11.21 508000
-
 recombinant His6-tagged enzyme, gel filtration Lactobacillus delbrueckii subsp. lactis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.7 Lactobacillus delbrueckii subsp. lactis F0HXE4
-
-
3.4.11.7 Lactobacillus delbrueckii subsp. lactis DSM 20072 F0HXE4
-
-
3.4.11.7 Lactococcus lactis ssp. lactis
-
-
-
3.4.11.7 Lactococcus lactis ssp. lactis DSM 20481
-
-
-
3.4.11.21 Lactobacillus delbrueckii subsp. lactis F0HXE4
-
-
3.4.11.21 Lactobacillus delbrueckii subsp. lactis DSM 20072 F0HXE4
-
-
3.4.11.21 Lactococcus lactis ssp. lactis
-
-
-
3.4.11.21 Lactococcus lactis ssp. lactis DSM 20481
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.7 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactococcus lactis ssp. lactis
3.4.11.7 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactobacillus delbrueckii subsp. lactis
3.4.11.21 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactococcus lactis ssp. lactis
3.4.11.21 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactobacillus delbrueckii subsp. lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.7 additional information the Lb-PepA activity decreases during the stationary growth phase, which is caused by degradation of the enzyme Lactobacillus delbrueckii subsp. lactis
-
3.4.11.21 additional information the Lb-PepA activity decreases during the stationary growth phase, which is caused by degradation of the enzyme Lactobacillus delbrueckii subsp. lactis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.7 L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Asp + 4-nitroaniline
-
 ?
3.4.11.7 L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Asp + 4-nitroaniline
-
 ?
3.4.11.7 L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Asp + 4-nitroaniline
-
 ?
3.4.11.7 L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Asp + 4-nitroaniline
-
 ?
3.4.11.7 L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Glu + 4-nitroaniline
-
 ?
3.4.11.7 L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Glu + 4-nitroaniline
-
 ?
3.4.11.7 L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Glu + 4-nitroaniline
-
 ?
3.4.11.7 L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Glu + 4-nitroaniline
-
 ?
3.4.11.21 L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Asp + 4-nitroaniline
-
 ?
3.4.11.21 L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Asp + 4-nitroaniline
-
 ?
3.4.11.21 L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Asp + 4-nitroaniline
-
 ?
3.4.11.21 L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Asp + 4-nitroaniline
-
 ?
3.4.11.21 L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Glu + 4-nitroaniline
-
 ?
3.4.11.21 L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Glu + 4-nitroaniline
-
 ?
3.4.11.21 L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Glu + 4-nitroaniline
-
 ?
3.4.11.21 L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Glu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.7 homododecamer 12 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 39400, about, sequence calculation Lactococcus lactis ssp. lactis
3.4.11.7 homododecamer 12 * 41000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 41100, about, sequence calculation Lactobacillus delbrueckii subsp. lactis
3.4.11.21 homododecamer 12 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 39400, about, sequence calculation Lactococcus lactis ssp. lactis
3.4.11.21 homododecamer 12 * 41000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 41100, about, sequence calculation Lactobacillus delbrueckii subsp. lactis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.7 glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactococcus lactis ssp. lactis
3.4.11.7 glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.7 Lb-PepA
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.7 Lc-PepA
-
 Lactococcus lactis ssp. lactis
3.4.11.7 More see also EC 3.4.11.21 Lactococcus lactis ssp. lactis
3.4.11.7 More see also EC 3.4.11.21 Lactobacillus delbrueckii subsp. lactis
3.4.11.7 PepA
-
 Lactococcus lactis ssp. lactis
3.4.11.7 PepA
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactococcus lactis ssp. lactis
3.4.11.21 glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 Lb-PepA
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 Lc-PepA
-
 Lactococcus lactis ssp. lactis
3.4.11.21 More see also EC 3.4.11.7 Lactococcus lactis ssp. lactis
3.4.11.21 More see also EC 3.4.11.7 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 PepA
-
 Lactococcus lactis ssp. lactis
3.4.11.21 PepA
-
 Lactobacillus delbrueckii subsp. lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.7 60
-
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.7 65
-
-
 Lactococcus lactis ssp. lactis
3.4.11.21 60
-
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 65
-
-
 Lactococcus lactis ssp. lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.7 10 80 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
3.4.11.7 20 75 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis
3.4.11.21 10 80 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
3.4.11.21 20 75 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.7
-
-
 purified recombinant His-tagged enzyme, completely stable for over 70 h Lactococcus lactis ssp. lactis
3.4.11.7
-
-
 purified recombinant His-tagged enzyme, over 80% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.7 40
-
 purified recombinant His-tagged enzyme, over 60% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.7 45
-
 purified recombinant His-tagged enzyme, half-life at 5°C is about 20 h, inactivation within 70 h Lactococcus lactis ssp. lactis
3.4.11.7 50
-
 purified recombinant His-tagged enzyme, over 15% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.7 55
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 4-5 h, inactivation within 65 h Lactococcus lactis ssp. lactis
3.4.11.7 60
-
 purified recombinant His-tagged enzyme, inactivation within 10 h Lactobacillus delbrueckii subsp. lactis
3.4.11.7 65
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 2 h, inactivation within 10 h Lactococcus lactis ssp. lactis
3.4.11.21
-
-
 purified recombinant His-tagged enzyme, completely stable for over 70 h Lactococcus lactis ssp. lactis
3.4.11.21
-
-
 purified recombinant His-tagged enzyme, over 80% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.21 40
-
 purified recombinant His-tagged enzyme, over 60% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.21 45
-
 purified recombinant His-tagged enzyme, half-life at 5°C is about 20 h, inactivation within 70 h Lactococcus lactis ssp. lactis
3.4.11.21 50
-
 purified recombinant His-tagged enzyme, over 15% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
3.4.11.21 55
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 4-5 h, inactivation within 65 h Lactococcus lactis ssp. lactis
3.4.11.21 60
-
 purified recombinant His-tagged enzyme, inactivation within 10 h Lactobacillus delbrueckii subsp. lactis
3.4.11.21 65
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 2 h, inactivation within 10 h Lactococcus lactis ssp. lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.7 6
-
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.7 8
-
-
 Lactococcus lactis ssp. lactis
3.4.11.21 6
-
-
 Lactobacillus delbrueckii subsp. lactis
3.4.11.21 8
-
-
 Lactococcus lactis ssp. lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.7 5 10 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
3.4.11.7 5 8 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis
3.4.11.21 5 10 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
3.4.11.21 5 8 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis