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Literature summary extracted from

  • Laurinyecz, B.; Vedelek, V.; Kovacs, A.L.; Szilasi, K.; Lipinszki, Z.; Slezak, C.; Darula, Z.; Juhasz, G.; Sinka, R.
    Sperm-leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm (2019), PLoS Genet., 15, e1007987 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(ET)S-Lap3MB01319, overview Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(ET1)S-Lap4MB11296, overview Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(MIC)S-Lap2MI14597, overview Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant S-Lap8KK106866, overview Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview. Despite the mitochondrial localization, S-Lap1resc-HA construct is not able to rescue the male sterile phenotype of the S-Lap1DELTA7 mutant, indicating that the C-terminal tag may modify the structure of the protein, which could prevent its normal function Drosophila melanogaster
3.4.11.1 additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview. Despite the mitochondrial localization, S-Lap1resc-HA construct is not able to rescue the male sterile phenotype of the S-Lap1DELTA7 mutant, indicating that the C-terminal tag may modify the structure of the protein, which could prevent its normal function. Strong reduction of S-Lap1-HAint in the pellet fraction of testis samples from the S-Lap1-HAint-containing S-Lap2MI14597, S-Lap3MB01319, S-Lap4MB11296, S-Lap5DELTA14, and S-Lap6MI06848 mutants, where the S-Lap1-HAint protein is present mainly in the supernatant, suggesting a problem with paracrystalline formation Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 mitochondrion two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The S-Lap enzymes are important constituents of the paracrystalline material in mitochondria of Drosophila melanogaster sperm. S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative, S-Lap proteins are collectively present in the isolates Drosophila melanogaster 5739
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mn2+ required Drosophila melanogaster
3.4.11.1 Zn2+ required Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Drosophila melanogaster Q500X4
-
-
3.4.11.1 Drosophila melanogaster Q7K2S9
-
-
3.4.11.1 Drosophila melanogaster Q7K5K9
-
-
3.4.11.1 Drosophila melanogaster Q8T4F1
-
-
3.4.11.1 Drosophila melanogaster Q95R35
-
-
3.4.11.1 Drosophila melanogaster Q961W5
-
-
3.4.11.1 Drosophila melanogaster Q9VSM6
-
-
3.4.11.1 Drosophila melanogaster Q9VSM7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 semen
-
Drosophila melanogaster
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3.4.11.1 testis
-
Drosophila melanogaster
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Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 55000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 leucylaminopeptidase
-
Drosophila melanogaster
3.4.11.1 S-Lap1
-
Drosophila melanogaster
3.4.11.1 S-Lap2
-
Drosophila melanogaster
3.4.11.1 S-Lap3
-
Drosophila melanogaster
3.4.11.1 S-Lap4
-
Drosophila melanogaster
3.4.11.1 S-Lap5
-
Drosophila melanogaster
3.4.11.1 S-Lap6
-
Drosophila melanogaster
3.4.11.1 S-Lap7
-
Drosophila melanogaster
3.4.11.1 S-Lap8
-
Drosophila melanogaster
3.4.11.1 sperm-leucylaminopeptidase
-
Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7.4
-
assay at Drosophila melanogaster

General Information

EC Number General Information Comment Organism
3.4.11.1 malfunction S-Lap1 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap2 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap3 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap4 gene mutant homozygotes are male semi-sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap5 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap6 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap7 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 malfunction S-Lap8 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
3.4.11.1 physiological function sperm-leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm Drosophila melanogaster