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Literature summary extracted from

  • Chopra, T.; Banerjee, S.; Gupta, S.; Yadav, G.; Anand, S.; Surolia, A.; Roy, R.P.; Mohanty, D.; Gokhale, R.S.
    Novel intermolecular iterative mechanism for biosynthesis of mycoketide catalyzed by a bimodular polyketide synthase (2008), PLoS Biol., 6, e163 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.295 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.295 C2226A inactive Mycobacterium tuberculosis
2.3.1.295 S2672A inactive Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.295 431000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.295 a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+ Mycobacterium tuberculosis
-
 a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
2.3.1.295 a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+ Mycobacterium tuberculosis H37Rv
-
 a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.295 Mycobacterium tuberculosis
-
-
-
2.3.1.295 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.295 Ni-NTA column chromatography and Resource-Q chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.295 9-hydroxy-decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 9-hydroxy-decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.3.1.295 a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
2.3.1.295 a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
2.3.1.295 decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 dodecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 dodecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.3.1.295 hexadecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 hexanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 hexanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.3.1.295 octadecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 octanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 octanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.3.1.295 propionyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.295 tetradecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.295 ? x * 212000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.295 pks12
-
 Mycobacterium tuberculosis
2.3.1.295 polyketide synthase 12
-
 Mycobacterium tuberculosis