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Literature summary extracted from
- Fructan active enzymes (FAZY) activities and biosynthesis of fructooligosaccharides in the vacuoles of Agave tequilana c variety plants of different age (2017), Planta, 245, 265-281 .
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.99 | vacuole | activities appeared in all plant developmental stages | Agave tequilana | 5773 | - |
| 2.4.1.100 | vacuole | activities appeared in all plant developmental stages | Agave tequilana | 5773 | - |
| 3.2.1.153 | protoplast | - |
Agave tequilana | - |
- |
| 3.2.1.153 | vacuole | - |
Agave tequilana | 5773 | - |
| 3.2.1.154 | protoplast | - |
Agave tequilana | - |
- |
| 3.2.1.154 | vacuole | - |
Agave tequilana | 5773 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.153 | fructan + H2O | Agave tequilana | Agave tequilana fructans | D-fructose + ? | - |
? |  |
| 3.2.1.153 | additional information | Agave tequilana | Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age | ? | - |
? | |
| 3.2.1.154 | fructan + H2O | Agave tequilana | Agave tequilana fructans | D-fructose + ? | - |
? | |
| 3.2.1.154 | additional information | Agave tequilana | Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.99 | Agave tequilana | A7RDD3 | - |
- |
| 2.4.1.99 | Agave tequilana Weber | A7RDD3 | - |
- |
| 2.4.1.100 | Agave tequilana | A7LJR5 | - |
- |
| 2.4.1.100 | Agave tequilana Weber | A7LJR5 | - |
- |
| 3.2.1.153 | Agave tequilana | - |
cv. Weber Blue | - |
| 3.2.1.154 | Agave tequilana | - |
cv. Weber Blue | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.153 | vacuole isolation from agave mesontle protoplasts | Agave tequilana |
| 3.2.1.154 | vacuole isolation from agave mesontle protoplasts | Agave tequilana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.100 | mesontle | - |
Agave tequilana | - |
| 3.2.1.153 | leaf base | - |
Agave tequilana | - |
| 3.2.1.153 | additional information | 7-year-old plants | Agave tequilana | - |
| 3.2.1.153 | stem | - |
Agave tequilana | - |
| 3.2.1.154 | leaf base | - |
Agave tequilana | - |
| 3.2.1.154 | additional information | 7-year-old plants | Agave tequilana | - |
| 3.2.1.154 | stem | - |
Agave tequilana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.99 | 2 1-kestose | - |
Agave tequilana | 1-nystose + ? | - |
? | |
| 2.4.1.99 | 2 1-kestose | - |
Agave tequilana Weber | 1-nystose + ? | - |
? | |
| 2.4.1.99 | 2 sucrose | beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose | Agave tequilana | D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside | - |
? | |
| 2.4.1.99 | 2 sucrose | beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose | Agave tequilana Weber | D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside | - |
? | |
| 2.4.1.100 | 2 1-kestose | - |
Agave tequilana | 1-nystose + ? | - |
? | |
| 2.4.1.100 | 2 1-kestose | - |
Agave tequilana Weber | 1-nystose + ? | - |
? | |
| 2.4.1.100 | 2 sucrose | beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose | Agave tequilana | D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside | - |
? | |
| 2.4.1.100 | 2 sucrose | beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose | Agave tequilana Weber | D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside | - |
? | |
| 3.2.1.153 | fructan + H2O | Agave tequilana fructans | Agave tequilana | D-fructose + ? | - |
? | |
| 3.2.1.153 | inulin + H2O | - |
Agave tequilana | D-fructose + ? | - |
? | |
| 3.2.1.153 | additional information | Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age | Agave tequilana | ? | - |
? | |
| 3.2.1.153 | additional information | hydrolysis of terminal, non-reducing 2,1-linked beta-D-fructofuranose residues in fructans | Agave tequilana | ? | - |
? | |
| 3.2.1.154 | fructan + H2O | Agave tequilana fructans | Agave tequilana | D-fructose + ? | - |
? | |
| 3.2.1.154 | inulin + H2O | - |
Agave tequilana | D-fructose + ? | - |
? | |
| 3.2.1.154 | additional information | Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age | Agave tequilana | ? | - |
? | |
| 3.2.1.154 | additional information | hydrolysis of terminal, non-reducing 2,6-linked beta-D-fructofuranose residues in fructans | Agave tequilana | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.99 | 1-SST | - |
Agave tequilana |
| 2.4.1.99 | sucrose:sucrose 1-fructosyltransferase | - |
Agave tequilana |
| 2.4.1.100 | 1-FFT | - |
Agave tequilana |
| 2.4.1.100 | fructan:fructan 1-fructosyltransferase | - |
Agave tequilana |
| 3.2.1.153 | 1-FEH | - |
Agave tequilana |
| 3.2.1.153 | FEH | - |
Agave tequilana |
| 3.2.1.153 | fructan 1-exohydrolase | - |
Agave tequilana |
| 3.2.1.153 | fructanexohydrolase | - |
Agave tequilana |
| 3.2.1.154 | 6-FEH | - |
Agave tequilana |
| 3.2.1.154 | FEH | - |
Agave tequilana |
| 3.2.1.154 | fructan 6-exohydrolase | - |
Agave tequilana |
| 3.2.1.154 | fructanexohydrolase | - |
Agave tequilana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.153 | 40 | - |
- |
Agave tequilana |
| 3.2.1.154 | 40 | - |
- |
Agave tequilana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.153 | 4.5 | - |
- |
Agave tequilana |
| 3.2.1.154 | 4.5 | - |
- |
Agave tequilana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.153 | metabolism | proposed model for the biosynthesis of fructooligosaccharides (FOSs) in Agave tequilana Weber Blue variety, overview | Agave tequilana |
| 3.2.1.153 | physiological function | fructan metabolism in Agave tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure, overview. Analysis of the specific activities of involved vacuolar fructan active enzymes (FAZY) in Agave tequilana plants of different age and the biosynthesis of fructooligosaccharides (FOSs). Fructan hydrolysis is carried out by FEH enzymes, that remove terminal fructosyl units from fructan chains, to fulfill a diverse set of functions in the plant, such as energy supply during plant growth, maintenance of the osmotic pressure in the vacuoles, and modulation of the oligofructans amounts under oxidative stress and freezing tolerance | Agave tequilana |
| 3.2.1.154 | metabolism | proposed model for the biosynthesis of fructooligosaccharides (FOSs) in Agave tequilana Weber Blue variety, overview | Agave tequilana |
| 3.2.1.154 | physiological function | fructan metabolism in Agave tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure, overview. Analysis of the specific activities of involved vacuolar fructan active enzymes (FAZY) in Agave tequilana plants of different age and the biosynthesis of fructooligosaccharides (FOSs). Fructan hydrolysis is carried out by FEH enzymes, that remove terminal fructosyl units from fructan chains, to fulfill a diverse set of functions in the plant, such as energy supply during plant growth, maintenance of the osmotic pressure in the vacuoles, and modulation of the oligofructans amounts under oxidative stress and freezing tolerance | Agave tequilana |
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Release 2023.1 (January 2023)
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