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Literature summary extracted from

  • Chou, J.; Kuleck, G.; Cohen, J.; Mulbry, W.
    Partial purification and characterization of an inducible indole-3-acetyl-L-aspartic acid hydrolase from Enterobacter agglomerans (1996), Plant Physiol., 112, 1281-1287 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.134 Cu2+ 1 mM, 60% inhibition in presence of Mg2+ Pantoea agglomerans
3.5.1.134 dithiothreitol 1 mM, 80% inhibition in presence of Mg2+ and 50% inhibition in absence of divalent cations Pantoea agglomerans
3.5.1.134 Triton X-100 1%, 50% inhibition in absence of divalent cations Pantoea agglomerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.134 13.5
-
 (indol-3-yl)acetyl-L-aspartate pH 7.0, 30°C Pantoea agglomerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.134 Mn2+ 1 mM, 40% increase in activity Pantoea agglomerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.134 40000 55000 gel filtration Pantoea agglomerans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.134 Pantoea agglomerans O50173
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.134 partial Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.134 (indol-3-yl)acetyl-L-aspartate + H2O
-
 Pantoea agglomerans (indol-3-yl)acetate + L-aspartate
-
 ?
3.5.1.134 additional information enzyme is highly specific for (indol-3-yl)acetyl-L-aspartate. No substrates: indole-3-acetic acid, ammonium sulfate, urea, indoleacetamide Pantoea agglomerans ?
-
 ?
3.5.1.134 N-acetyl-L-aspartate + H2O
-
 Pantoea agglomerans acetate + L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.134 monomer 1 * 45000, SDS-PAGE Pantoea agglomerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.134 8 8.5
-
 Pantoea agglomerans

Expression

EC Number Organism Comment Expression
3.5.1.134 Pantoea agglomerans expression is induced by growth on (indol-3-yl)acetyl-L-aspartate or N-acetyl-L-aspartate up