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Literature summary extracted from
- Classic myrosinase-dependent degradation of indole glucosinolate attenuates fumonisin B1-induced programmed cell death in Arabidopsis (2015), Plant J., 81, 920-933 .
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.147 | additional information | mutant pen2-2, the mutant of atypical myrosinase PEN2 with highly reduced enzyme activity, shows fewer lesions compared with myrosinase double mutant tgg1 tgg2 and exhibits a symptoms similar to the wild-type in response to mycotoxin fumonisin B1 (FB1), indicating that deficiency of TGG instead of PEN2 renders plants significantly more sensitive to FB1 | Arabidopsis thaliana |
| 3.2.1.147 | additional information | the costructed tgg1 tgg2 double mutant, which has greatly reduced TGG activity, shows more severe lesion formation and cell death symptoms than Col-0. Mutant pen2-2, the mutant of atypical myrosinase PEN2 with highly reduced enzyme activity, shows fewer lesions compared with myrosinase double mutant tgg1 tgg2 and exhibits a symptoms similar to the wild-type in response to mycotoxin fumonisin B1 (FB1), indicating that deficiency of TGG instead of PEN2 renders plants significantly more sensitive to FB1 | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.147 | peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.147 | 1-methoxyindol-3-ylmethyl-glucosinolate + H2O | Arabidopsis thaliana | - |
1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? |  |
| 3.2.1.147 | 1-methoxyindol-3-ylmethyl-glucosinolate + H2O | Arabidopsis thaliana Col-0 | - |
1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | 4-methoxyindol-3-ylmethyl-glucosinolate + H2O | Arabidopsis thaliana | - |
4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | 4-methoxyindol-3-ylmethyl-glucosinolate + H2O | Arabidopsis thaliana Col-0 | - |
4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | indol-3-ylmethyl glucosinolate + H2O | Arabidopsis thaliana | - |
indol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | indol-3-ylmethyl glucosinolate + H2O | Arabidopsis thaliana Col-0 | - |
indol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.147 | Arabidopsis thaliana | O64883 | - |
- |
| 3.2.1.147 | Arabidopsis thaliana | P37702 | - |
- |
| 3.2.1.147 | Arabidopsis thaliana Col-0 | O64883 | - |
- |
| 3.2.1.147 | Arabidopsis thaliana Col-0 | P37702 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.147 | leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.147 | 1-methoxyindol-3-ylmethyl-glucosinolate + H2O | - |
Arabidopsis thaliana | 1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | 1-methoxyindol-3-ylmethyl-glucosinolate + H2O | - |
Arabidopsis thaliana Col-0 | 1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | 4-methoxyindol-3-ylmethyl-glucosinolate + H2O | - |
Arabidopsis thaliana | 4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | 4-methoxyindol-3-ylmethyl-glucosinolate + H2O | - |
Arabidopsis thaliana Col-0 | 4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | indol-3-ylmethyl glucosinolate + H2O | - |
Arabidopsis thaliana | indol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | indol-3-ylmethyl glucosinolate + H2O | - |
Arabidopsis thaliana Col-0 | indol-3-ylmethyl isothiocyanate + D-glucose | - |
? | |
| 3.2.1.147 | additional information | the enzyme is active with indole and aliphatic glucosinolates | Arabidopsis thaliana | ? | - |
? | |
| 3.2.1.147 | additional information | the enzyme is active with indole and aliphatic glucosinolates | Arabidopsis thaliana Col-0 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.147 | AtBGLU38 | - |
Arabidopsis thaliana |
| 3.2.1.147 | atypical myrosinase | - |
Arabidopsis thaliana |
| 3.2.1.147 | beta-glucosidase 26, peroxisomal | UniProt | Arabidopsis thaliana |
| 3.2.1.147 | beta-glucosidase 38 | - |
Arabidopsis thaliana |
| 3.2.1.147 | BGLU26 | - |
Arabidopsis thaliana |
| 3.2.1.147 | myrosinase | - |
Arabidopsis thaliana |
| 3.2.1.147 | PEN2 | - |
Arabidopsis thaliana |
| 3.2.1.147 | sinigrinase 1 | - |
Arabidopsis thaliana |
| 3.2.1.147 | TGG1 | - |
Arabidopsis thaliana |
| 3.2.1.147 | thioglucosidase 1 | - |
Arabidopsis thaliana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.147 | malfunction | the classic myrosinase beta-thioglucoside glucohydrolase (TGG)-deficient double mutant tgg1 tgg2, rather than atypical myrosinase-deficient mutant pen2-2, is more sensitive to the mycotoxin fumonisin B1 (FB1) than wild-type Col-0, and the elevated expression of isozyme TGG1, but not of PEN2, correlates with the decrease in indole glucosinolate (IGS), TGG-dependent IGS hydrolysis is involved in FB1-induced programmed cell death (PCD) | Arabidopsis thaliana |
| 3.2.1.147 | metabolism | metabolism of tryptophan-derived indole glucosinolate (IGS), camalexin and indol-3-acetic acid (IAA) as well as methioninederived aliphatic glucosinolate (AGS) in Arabidopsis thaliana involving the myrosinase enzymes TGG1 and PEN2, overview | Arabidopsis thaliana |
| 3.2.1.147 | metabolism | metabolism of tryptophan-derived indole glucosinolate (IGS), camalexin and indole-3-acetic acid (IAA) as well as methioninederived aliphatic glucosinolate (AGS) in Arabidopsis thaliana involving the myrosinase enzymes TGG1 and PEN2, overview. FB1-induced biosynthesis of glucosinolates. FB1 treatment triggers not only biosynthesis but also hydrolysis of IGS | Arabidopsis thaliana |
| 3.2.1.147 | additional information | the cyp79B2 cyp79B3 mutant, which has a greatly reduced level of indole glucosinolates, is more sensitive to mycotoxin fumonisin B1 (FB1) | Arabidopsis thaliana |
| 3.2.1.147 | physiological function | classical TGG-dependent hydrolysis of indole glucosinolate (IGS) restricts FB1-induced programmed cell death (PCD). Mycotoxin fumonisin B1 (FB1) causes the accumulation of reactive oxygen species (ROS) which then leads to PCD in Arabidopsis thaliana. FB1-induced biosynthesis of glucosinolates. FB1 treatment triggers not only biosynthesis but also hydrolysis of IGS | Arabidopsis thaliana |
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