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Literature summary extracted from

  • Leonhardt, N.; Divol, F.; Chiarenza, S.; Deschamps, S.; Renaud, J.; Giacalone, C.; Laurent, N.; Berthome, R.; Peret, B.
    Tissue-specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology (2019), Plant J., 101, 731-741 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.9 molecular biology engineering the yeast fluorocytosine deaminase (FCY1) gene by creating a fusion with the bacterial uracil phosphoribosyl transferase (UPP) gene results in a recombinant protein that converts the precursor 5-fluorocytosine (5-FC) into 5-fluorouracyl, a drug used in the treatment of a range of cancers, which triggers DNA and RNA damage. The tissue-specific FCY-UPP system is a great tool to inactivate cells in a precise spatial and temporal manner, method evaluation, overview Escherichia coli
3.5.4.1 agriculture construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 gene upp, recombinant overexpression of 35S:FCY-UPP and pMYB60:FCY-UPP constructs in Arabdopsis thaliana ecotype Col-0, which affects the effect of 5-fluorouracil (5-FC) on the plants compared to wild-type plants, analysis of the tissue specificity of the FCY-UPP tandem, overview. The enzyme tandem is expressed in the xylem pole pericycle cells from which lateral roots are being formed. Expressing FCYUPP in the lateral root cap and epidermis using the J0951 enhancer trap line results in a strong reduction of primary root growth. Similarly, when FCY-UPP proteins are expressed in the guard cells using the pMYB60 promoter, no reduction in total leaf area is observed. These observations suggest that both the FCY-UPP tandem protein and the products of 5-FC incorporation remain in the cells where the construct is expressed, at least on a short time-scale. A tagged version of FCY-UPP fused to the GFP reporter is expressed under the epidermis-specific promoter of WEREWOLF Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.9 additional information engineering the yeast fluorocytosine deaminase (FCY1) gene by creating a fusion with the bacterial uracil phosphoribosyl transferase (UPP) gene results in a recombinant protein that converts the precursor 5-fluorocytosine (5-FC) into 5-fluorouracyl, a drug used in the treatment of a range of cancers, which triggers DNA and RNA damage. The FCY-UPP gene construct is expressed in specific cell types using enhancer trap lines and promoters, demonstrating that this marker acts in a cell-autonomous manner. It can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. A role for the lateral root cap and the epidermis in controlling root growth, a faster response, is also revealed. The 5-FC precursor acts systemically, as demonstrated by its ability to inhibit stomatal movements when supplied to the roots in combination with a guard cell-specific promoter. The tissular inactivation is reversible, and can therefore be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages Escherichia coli
3.5.4.1 additional information construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 UMP + diphosphate Escherichia coli
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Escherichia coli P0A8F0
-
-
3.5.4.1 Saccharomyces cerevisiae Q12178
-
-
3.5.4.1 Saccharomyces cerevisiae ATCC 204508 Q12178
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 UMP + diphosphate
-
 Escherichia coli uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
3.5.4.1 5-fluorocytosine + H2O
-
 Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
3.5.4.1 5-fluorocytosine + H2O
-
 Saccharomyces cerevisiae ATCC 204508 5-fluorouracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPP
-
 Escherichia coli
2.4.2.9 uracil phosphoribosyl transferase
-
 Escherichia coli