Literature summary extracted from

Nourrisson, C.; Wawrzyniak, I.; Cian, A.; Livrelli, V.; Viscogliosi, E.; Delbac, F.; Poirier, P.
On Blastocystis secreted cysteine proteases a legumain-activated cathepsin B increases paracellular permeability of intestinal Caco-2 cell monolayers (2016), Parasitology, 143, 1713-1722 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.34	expression in Escherichia coli	Blastocystis hominis

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.22.34	40000	-	SDS-PAGE	Blastocystis hominis
3.4.22.34	45200	-	calculated	Blastocystis hominis

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.34	Blastocystis hominis	D8M176	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.22.34	proteolytic modification	activation of the recombinant legumain is autocatalytic and triggered by acidic pH	Blastocystis hominis

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.34	benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O	-	Blastocystis hominis	benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.22.34	3	4	-	Blastocystis hominis

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Release 2023.1 (January 2023)