Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • White-Gilbertson, S.; Mullen, T.; Senkal, C.; Lu, P.; Ogretmen, B.; Obeid, L.; Voelkel-Johnson, C.
    Ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells (2009), Oncogene, 28, 1132-1141 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.24 gene CERS6, recombinant expression of N-terminally FLAG-tagged CerS6 and EGFP-tagged CerS6 in SW-480 cells and localization in the perinuclear region. When cells transfected with pCerS6-IRES2-EGFP are treated with TRAIL, GFP positive cells appear to be undergoing apoptosis. Cells stably transfected with CerS6 but not with the empty plasmid acquire a TRAIL sensitive phenotype Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 endoplasmic reticulum membrane
-
 Homo sapiens 5789
-
2.3.1.24 perinuclear space recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA Homo sapiens
-
 N-palmitoylsphinganine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Homo sapiens Q6ZMG9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 colonic cancer cell
-
 Homo sapiens
-
2.3.1.24 intestine high expression level of CerS6 Homo sapiens
-
2.3.1.24 SW-480 cell
-
 Homo sapiens
-
2.3.1.24 SW-620 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Homo sapiens N-palmitoylsphinganine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase 6
-
 Homo sapiens
2.3.1.24 CerS6
-
 Homo sapiens
2.3.1.24 Lass6
-
 Homo sapiens
2.3.1.24 longevity assurance homolog 6
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.24 malfunction RNAi against CerS6 results in a specific decrease in intracellular C16-ceramide and protects SW-480 cells against TRAIL-mediated apoptosis while increasing CerS6 expression sensitizes SW-620 cells to TRAIL. Downregulation of CerS6 does not interfere with caspase activation but appears to inhibit translocation of activated caspase-3 into the nucleus Homo sapiens
2.3.1.24 additional information recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region Homo sapiens
2.3.1.24 physiological function ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells. Ceramide synthase 6 (CerS6 also known as longevity assurance homolog 6/LASS6) preferentially generates C16-ceramide and can influence TRAIL susceptibility. CerS6 may regulate events at the nuclear membrane and allow late stage apoptotic signaling Homo sapiens