Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pao, K.C.; Wood, N.T.; Knebel, A.; Rafie, K.; Stanley, M.; Mabbitt, P.D.; Sundaramoorthy, R.; Hofmann, K.; van Aalten, D.M.F.; Virdee, S.
    Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity (2018), Nature, 556, 381-385 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.33
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.33 to 1.75 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.33 C4520 mutation impairs thioester-linked E3-Ub intermediate formation Homo sapiens
2.3.2.33 C4520A/C4572S loss of activity Homo sapiens
2.3.2.33 C4572S mutant retains activity and is able to form a discrete mono-ubiquitin adduct Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.33 Zn2+ a globular domain (residues 4501-4638) binds four Zn ions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.33 additional information Homo sapiens MYCBP2 contains two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. It shows esterification activity and intrinsic selectivity for threonine over serine ?
-
 ?
2.3.2.33 [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine Homo sapiens
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?
2.3.2.33 [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine Homo sapiens
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
-
 ?
2.3.2.33 [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine Homo sapiens
-
 [RCR-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.33 Homo sapiens O75592
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.33 neuron neuron-associated Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.33 additional information MYCBP2 contains two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. It shows esterification activity and intrinsic selectivity for threonine over serine Homo sapiens ?
-
 ?
2.3.2.33 [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine
-
 Homo sapiens [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?
2.3.2.33 [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine
-
 Homo sapiens [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
-
 ?
2.3.2.33 [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine
-
 Homo sapiens [RCR-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.33 E3 ubiquitin-protein ligase
-
 Homo sapiens
2.3.2.33 MYCBP2
-
 Homo sapiens
2.3.2.33 PHR1
-
 Homo sapiens