EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.11.64 | in complex with glutarate, succinate, and the inhibitor N-oxalyl-glycine | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.64 | (S)-2-hydroxyglutarate | weak product inhibition | Escherichia coli | |
1.14.11.64 | N-oxalyl-glycine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.64 | 0.1 | - |
2-oxoglutarate | pH 7.2, 30°C | Escherichia coli | |
1.14.11.64 | 0.65 | - |
Glutarate | pH 7.2, 30°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.13 | cytoplasmic membrane | - |
Escherichia coli | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.13 | L-2-hydroxyglutarate + a quinone | Escherichia coli | - |
2-oxoglutarate + a quinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.13 | Escherichia coli | - |
- |
- |
1.14.11.64 | Escherichia coli | P76621 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.13 | Ni-NTA column chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.11.64 | 0.053 | - |
pH 7.2, 30°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.13 | L-2-hydroxyglutarate + a quinone | - |
Escherichia coli | 2-oxoglutarate + a quinol | - |
? | |
1.1.5.13 | L-2-hydroxyglutarate + menaquinone-4 | - |
Escherichia coli | 2-oxoglutarate + menaquinol-4 | - |
? | |
1.1.5.13 | L-2-hydroxyglutarate + ubiquinone-1 | - |
Escherichia coli | 2-oxoglutarate + ubiquinol-1 | - |
? | |
1.14.11.64 | glutarate + 2-oxoglutarate + O2 | - |
Escherichia coli | (S)-2-hydroxyglutarate + succinate + CO2 | - |
? | |
1.14.11.64 | additional information | no substrates: oxalate, malonate, succinate, adipate, and pimelate | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.13 | L-2-hydroxyglutarate dehydrogenase | - |
Escherichia coli |
1.1.5.13 | L2HG dehydrogenase | - |
Escherichia coli |
1.1.5.13 | L2HG:quinone oxidoreductase | - |
Escherichia coli |
1.1.5.13 | lhgO | - |
Escherichia coli |
1.14.11.64 | csiD | - |
Escherichia coli |
1.14.11.64 | GlaH | - |
Escherichia coli |
1.14.11.64 | glutarate 2-hydroxylase | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.13 | FAD | dependent on | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.64 | physiological function | during catabolism of lysine to succinate, CsiD acts as an 2-oxoglutarate-dependent dioxygenase catalysing hydroxylation of glutarate to L-2-hydroxyglutarate. Repression of the pathway by CsiR is relieved upon glutarate binding. In a knockout strain, with carbon starvation and entry into the stationary phase, the intracellular concentration of glutarate accumulates to much higher levels than compared to the wild-type | Escherichia coli |