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Literature summary extracted from

  • Hai, Y.; Shinsky, S.A.; Porter, N.J.; Christianson, D.W.
    Histone deacetylase 10 structure and molecular function as a polyamine deacetylase (2017), Nat. Commun., 8, 15368 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.48 gene hdac10, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of TEV-cleavable N-terminal His-MBP-tagged enzyme from codon-optimized HDAC10 gene (residues 2-675) in Escherichia coli strain BL21(DE3), expression of wild-type and mutant enzymes Danio rerio
3.5.1.48 gene HDAC10, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of TEV-cleavable N-terminal His-MBP-tagged enzyme from codon-optimized HDAC10 gene in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.48 purified enzyme zHDAC10 mutant Y307F complexed with a polyamine transition state analogue inhibitor AAT, sitting drop vapour diffusion method, mixing of 400 nl of 30 mg/ml Y307F zHDAC10DELTA-AAT complex and 5 mM AAT with 400 nl of precipitant solution containing 0.2 M KH2PO4, and 20% PEG 3350, and equilibration against 0.08 ml precipitant solution, 24 h, 4°C, X-ray diffraction structure determination and analysis at 2.85 A resolution, molecular replacement using structure of the zHDAC6 CD1-TSA complex (PDB ID 5EEF) Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.48 D94A site-directed mutagenesis, steady-state kinetics Danio rerio
3.5.1.48 E274L site-directed mutagenesis, steady-state kinetics, mutation of the glutamate gatekeeper residue Danio rerio
3.5.1.48 additional information construction of deletion mutant DELTAnuA2, steady-state kinetics. The universal 310 helix nuA2 insertion in loop L1 further constricts the HDAC10 active site. Generation of mutant zHDAC10DELTA by treatement with trypsin Danio rerio
3.5.1.48 N93A site-directed mutagenesis, steady-state kinetics Danio rerio
3.5.1.48 Y307F site-directed mutagenesis, introduction of the deactivating Y307F mutation facilitates crystallization Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.48 7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one AAT, enzyme-bound structure analysis, overview. This inhibitor binds as a transition state analogue Danio rerio
3.5.1.48 7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one AAT, the inhibitor binds as a transition state analogue Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.48 additional information
-
 additional information steady-state kinetics Homo sapiens
3.5.1.48 0.01
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.017
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.022
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.05
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.06
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.06
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.06
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.06
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.066
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.07
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.07
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.08
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.09
-
 Acetylcadaverine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.1
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.11
-
 Acetylcadaverine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.11
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.11
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.11
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.13
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.13
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.13
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.14
-
 Nalpha,Nepsilon-diacetyl-L-lysinamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.14
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.15
-
 N1,N8-diacetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.15
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.15
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.16
-
 acetylputrescine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.16
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.16
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.17
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.17
-
 acetylputrescine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.18
-
 N1,N8-diacetylspermidine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.18
-
 N1-acetylspermine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.26
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.27
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.9
-
 N-butylacetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.48 Zn2+ catalytic zinc ion, enzyme binding structure, overview Homo sapiens
3.5.1.48 Zn2+ catalytic zinc ion, enzyme binding structure, overview Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.48 N8-acetylspermidine + H2O Homo sapiens
-
 acetate + spermidine
-
 ?
3.5.1.48 N8-acetylspermidine + H2O Danio rerio
-
 acetate + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.48 Danio rerio F1QCV2
-
-
3.5.1.48 Homo sapiens Q969S8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.48 recombinant TEV-cleavable N-terminal His-MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography and anion exchange chromatography, the MBP-tag is not cleaved off Homo sapiens
3.5.1.48 recombinant TEV-cleavable N-terminal His-MBP-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, TEV protease is used to remove the His-MBP-tag, followed by anion exchange chromatography and gel filtration Danio rerio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.48 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin + H2O very low activity Danio rerio ?
-
 ?
3.5.1.48 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin + H2O low activity Homo sapiens ?
-
 ?
3.5.1.48 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin + H2O very low activity Danio rerio ?
-
 ?
3.5.1.48 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin + H2O low activity Homo sapiens ?
-
 ?
3.5.1.48 acetylcadaverine + H2O high acitivity Homo sapiens acetate + cadaverine
-
 ?
3.5.1.48 acetylcadaverine + H2O high acitivity Danio rerio acetate + cadaverine
-
 ?
3.5.1.48 acetylputrescine + H2O high acitivity Homo sapiens acetate + putrescine
-
 ?
3.5.1.48 acetylputrescine + H2O high acitivity Danio rerio acetate + putrescine
-
 ?
3.5.1.48 additional information the conserved glutamate gatekeeper and a sterically constricted active site confer specificity for N8-acetylspermidine hydrolysis and disfavour acetyllysine hydrolysis. Analysis of the intermolecular interactions of N8-acetylspermidine in the active site of hHDAC10 based on the structure of the Danio rerio enzyme-inhibitor crystal structure zHDAC10-AAT complex. No activity with N-(3-aminopropyl)acetamide, TK(ac)PIW, AK(ac)P, GAK(ac), AK(ac), K(ac)NL, K(ac)NL, and GAK(ac)NLQ Homo sapiens ?
-
 ?
3.5.1.48 additional information zebrafish HDAC10 complexed with a transition-state analogue inhibitor reveals that a glutamate gatekeeper and a sterically constricted active site confer specificity for N8-acetylspermidine hydrolysis and disfavour acetyllysine hydrolysis. The N4 amino group of N8-acetylspermidine is recognized by a direct hydrogen bond with E117. No activity with TK(ac)PIW, AK(ac)P, GAK(ac), AK(ac), AK(ac)A, K(ac)NL, K(ac)NL, and GAK(ac)NLQ Danio rerio ?
-
 ?
3.5.1.48 N-(3-aminopropyl)acetamide + H2O very low activity Danio rerio propane-1,3-diamine + acetate
-
 ?
3.5.1.48 N-(8-aminooctyl)acetamide + H2O moderate activity Homo sapiens octane-1,8-diamine + acetate
-
 ?
3.5.1.48 N-(8-aminooctyl)acetamide + H2O moderate to high activity Danio rerio octane-1,8-diamine + acetate
-
 ?
3.5.1.48 N-butylacetamide + H2O very low activity Danio rerio butan-1-amine + acetate
-
 ?
3.5.1.48 N-butylacetamide + H2O low activity Homo sapiens butan-1-amine + acetate
-
 ?
3.5.1.48 N1,N8-diacetylspermidine + 2 H2O moderate activity Homo sapiens 2 acetate + spermidine
-
 ?
3.5.1.48 N1,N8-diacetylspermidine + H2O high activity Danio rerio 2 acetate + spermidine
-
 ?
3.5.1.48 N1-acetylspermidine + H2O very low activity Danio rerio acetate + spermidine
-
 ?
3.5.1.48 N1-acetylspermine + H2O very low activity Danio rerio acetate + spermine
-
 ?
3.5.1.48 N1-acetylspermine + H2O low activity Homo sapiens acetate + spermine
-
 ?
3.5.1.48 N8-acetylspermidine + H2O
-
 Homo sapiens acetate + spermidine
-
 ?
3.5.1.48 N8-acetylspermidine + H2O
-
 Danio rerio acetate + spermidine
-
 ?
3.5.1.48 N8-acetylspermidine + H2O best substrate Homo sapiens acetate + spermidine
-
 ?
3.5.1.48 N8-acetylspermidine + H2O best substrate Danio rerio acetate + spermidine
-
 ?
3.5.1.48 Nalpha,Nepsilon-diacetyl-L-lysinamide + H2O low activity Homo sapiens ?
-
 ?
3.5.1.48 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide + H2O low activity Homo sapiens ?
-
 ?
3.5.1.48 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide + H2O low activity Danio rerio ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.48 HDAC10
-
 Homo sapiens
3.5.1.48 HDAC10
-
 Danio rerio
3.5.1.48 histone deacetylase 10
-
 Homo sapiens
3.5.1.48 histone deacetylase 10
-
 Danio rerio
3.5.1.48 zHDAC10
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.48 22
-
 assay at room temperature Homo sapiens
3.5.1.48 22
-
 assay at room temperature Danio rerio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.48 additional information
-
 additional information steady-state kinetics of wild-type and mutant enzymes Danio rerio
3.5.1.48 0.00007
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.00033
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.00038
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.00046
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.0006
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.0013
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.0013
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.0015
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.008
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.0092
-
 Nalpha,Nepsilon-diacetyl-L-lysinamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.011
-
 N1-acetylspermine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.013
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.02
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.021
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.028
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.034
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.051
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.053
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.06
-
 N-butylacetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.082
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.1
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.123
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.126
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.14
-
 Acetylcadaverine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.14
-
 N1,N8-diacetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.21
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.21
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.24
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.28
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.29
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.35
-
 acetylputrescine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.37
-
 Acetylcadaverine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.48
-
 N1,N8-diacetylspermidine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.58
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.7
-
 acetylputrescine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.9
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant D94A Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.48 8
-
 assay at Homo sapiens
3.5.1.48 8
-
 assay at Danio rerio

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.48 0.0007
-
 7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.0007
-
 7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio

General Information

EC Number General Information Comment Organism
3.5.1.48 physiological function cationic polyamines such as spermidine and spermine are critical in all forms of life, as they regulate the function of biological macromolecules. Intracellular polyamine metabolism is regulated by reversible acetylation and dysregulated polyamine metabolism is associated with neoplastic diseases such as colon cancer, prostate cancer and neuroblastoma. Both HDAC10 and its product spermidine are known to promote cellular survival through autophagy Homo sapiens
3.5.1.48 physiological function Cationic polyamines such as spermidine and spermine are critical in all forms of life, as they regulate the function of biological macromolecules. Intracellular polyamine metabolism is regulated by reversible acetylation. Both HDAC10 and its product spermidine are known to promote cellular survival through autophagy Danio rerio

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.48 0.0012
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.0087
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.01
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.012
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.015
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.023
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.027
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 0.038
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.061
-
 N1-acetylspermine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.066
-
 Nalpha,Nepsilon-diacetyl-L-lysinamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.067
-
 N-butylacetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.072
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.14
-
 N1,N8-diacetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.163
-
 Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 0.213
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.215
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.304
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.333
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.35
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 Danio rerio
3.5.1.48 0.757
-
 7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio
3.5.1.48 0.82
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 0.93
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 0.97
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 1.02
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant N93A Danio rerio
3.5.1.48 1.16
-
 Acetylcadaverine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 1.24
-
 N-(8-aminooctyl)acetamide pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 1.27
-
 Acetylcadaverine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 2.06
-
 acetylputrescine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 2.18
-
 acetylputrescine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 2.8
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant enzyme Homo sapiens
3.5.1.48 4.11
-
 Acetylcadaverine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 4.375
-
 acetylputrescine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 4.46
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant wild-type enzyme Danio rerio
3.5.1.48 6.43
-
 N8-Acetylspermidine pH 8.0, 22°C, recombinant mutant D94A Danio rerio
3.5.1.48 8.84
-
 7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin pH 8.0, 22°C, recombinant mutant E274L Danio rerio