Literature summary extracted from

Grishin, A.V.; Lavrova, N.V.; Lyashchuk, A.M.; Strukova, N.V.; Generalova, M.S.; Ryazanova, A.V.; Shestak, N.V.; Boksha, I.S.; Polyakov, N.B.; Galushkina, Z.M.; Soboleva, L.A.; Vetchinin, S.S.; Pavlov, V.M.; Karyagina, A.S.; Lunin, V.G.
The influence of dimerization on the pharmacokinetics and activity of an antibacterial enzyme lysostaphin (2019), Molecules, 24, 1879 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.24.75	expressed in Escherichia coli M15 cells	Staphylococcus simulans

General Stability

EC Number	General Stability	Organism
3.4.24.75	lysostaphin fused it with an anti-parallel alpha-helical dimerization domain has a more favorable pharmacokinetic profile with increased terminal half-life compared to monomeric lysostaphin. However, the staphylolytic activity of dimerized lysostaphin is decreased.	Staphylococcus simulans

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.24.75	Staphylococcus aureus cells + H2O	Staphylococcus simulans	Staphylococcus aureus ATCC 29213	fragments of elastin	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.24.75	Staphylococcus simulans	P10547	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.24.75	WorkBeads 40S cation exchange column chromatography	Staphylococcus simulans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.24.75	pentaglycine + H2O	pentaglycine peptide sc-471644A	Staphylococcus simulans	?	-	?
3.4.24.75	Staphylococcus aureus cells + H2O	Staphylococcus aureus ATCC 29213	Staphylococcus simulans	fragments of elastin	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.24.75	?	x * 29000, SDS-PAGE	Staphylococcus simulans
3.4.24.75	?	x * 45000, lysostaphin fused it with an anti-parallel alpha-helical dimerization domain, SDS-PAGE	Staphylococcus simulans

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Release 2023.1 (January 2023)