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Literature summary extracted from

  • Cui, C.; Kim, D.; Jung, S.; Kim, S.; Im, W.
    Enhanced production of gypenoside LXXV using a novel ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property (2017), Molecules, 22, 844-858 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.191 synthesis the enzyme is applied for the production of gypenoside LXXV from gypenoside XVII at the gram-scale. The pure GypLXXV can be used for the study of anti-cancer effects against three kinds of cancer cells in vitro Microbacterium sp.
3.2.1.194 synthesis the enzyme is applied for the production of gypenoside LXXV from gypenoside XVII at the gram-scale. The pure GypLXXV can be used for the study of anti-cancer effects against three kinds of cancer cells in vitro Microbacterium sp. Gsoil167

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.191 BglG167b cloning and overexpression in Escherichia coli as GST-tagged enzyme, phylogenetic analysis of BglG167b Microbacterium sp.
3.2.1.194 BglG167b cloning and overexpression in Escherichia coli as GST-tagged enzyme, phylogenetic analysis of BglG167b Microbacterium sp. Gsoil167

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.191 Ca2+
-
 Microbacterium sp.
3.2.1.191 Co2+
-
 Microbacterium sp.
3.2.1.191 Cu2+
-
 Microbacterium sp.
3.2.1.191 EDTA
-
 Microbacterium sp.
3.2.1.191 SDS
-
 Microbacterium sp.
3.2.1.194 Ca2+
-
 Microbacterium sp. Gsoil167
3.2.1.194 Co2+
-
 Microbacterium sp. Gsoil167
3.2.1.194 Cu2+
-
 Microbacterium sp. Gsoil167
3.2.1.194 EDTA
-
 Microbacterium sp. Gsoil167
3.2.1.194 SDS
-
 Microbacterium sp. Gsoil167

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.191 additional information the enzyme activity is not affected by Na+, K+, Mg2+, and Mn2+ Microbacterium sp.
3.2.1.194 additional information the enzyme activity is not affected by Na+, K+, Mg2+, and Mn2+ Microbacterium sp. Gsoil167

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.191 ginsenoside 20(S)-Rg3 + H2O Microbacterium sp.
-
 ginsenoside 20(S)-Rh2 + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside F2 + H2O Microbacterium sp.
-
 ginsenoside C-K + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside F2 + H2O Microbacterium sp. Gsoil 167
-
 ginsenoside C-K + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rb2 + H2O Microbacterium sp.
-
 ginsenoside C-O + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rb2 + H2O Microbacterium sp. Gsoil 167
-
 ginsenoside C-O + D-glucopyranose
-
 ?
3.2.1.191 gypenoside XVII + H2O Microbacterium sp.
-
 gypenoside LXXV + D-glucose
-
 ?
3.2.1.194 ginsenoside Re + H2O Microbacterium sp. Gsoil167
-
 ginsenoside Rg1 + L-rhamnose
-
 ?
3.2.1.194 ginsenoside Rg1 + H2O Microbacterium sp. Gsoil167
-
 ginsenoside F1 + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.191 Microbacterium sp. L0EKQ7 from ginseng-cultivating soil
-
3.2.1.191 Microbacterium sp. Gsoil 167 L0EKQ7 from ginseng-cultivating soil
-
3.2.1.194 Microbacterium sp. Gsoil167 L0EKQ7 from ginseng-cultivating soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.191 recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Microbacterium sp.
3.2.1.194 recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Microbacterium sp. Gsoil167

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.191 2-nitrophenyl beta-D-glucopyranoside + H2O low activity Microbacterium sp. 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 2-nitrophenyl beta-D-glucopyranoside + H2O low activity Microbacterium sp. Gsoil 167 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 4-nitrophenyl alpha-D-glucopyranoside + H2O very low activity Microbacterium sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Microbacterium sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Microbacterium sp. Gsoil 167 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.191 ginsenoside 20(S)-Rg3 + H2O
-
 Microbacterium sp. ginsenoside 20(S)-Rh2 + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside F2 + H2O
-
 Microbacterium sp. ginsenoside C-K + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside F2 + H2O
-
 Microbacterium sp. Gsoil 167 ginsenoside C-K + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rb2 + H2O
-
 Microbacterium sp. ginsenoside C-O + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rb2 + H2O
-
 Microbacterium sp. Gsoil 167 ginsenoside C-O + D-glucopyranose
-
 ?
3.2.1.191 gypenoside XVII + H2O
-
 Microbacterium sp. gypenoside LXXV + D-glucose
-
 ?
3.2.1.191 additional information enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 Microbacterium sp. ?
-
 ?
3.2.1.191 additional information enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 Microbacterium sp. Gsoil 167 ?
-
 ?
3.2.1.194 ginsenoside Re + H2O
-
 Microbacterium sp. Gsoil167 ginsenoside Rg1 + L-rhamnose
-
 ?
3.2.1.194 ginsenoside Rg1 + H2O
-
 Microbacterium sp. Gsoil167 ginsenoside F1 + D-glucose
-
 ?
3.2.1.194 additional information enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 Microbacterium sp. Gsoil167 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.191 ? x * 90300, about, sequence calculation, x * 116000, about, recombinant GST-tagged enzyme BglG167b, sequence calculation Microbacterium sp.
3.2.1.194 ? x * 90300, about, sequence calculation, x * 116000, about, recombinant GST-tagged enzyme BglG167b, sequence calculation Microbacterium sp. Gsoil167

Synonyms

EC Number Synonyms Comment Organism
3.2.1.191 BglG167b
-
 Microbacterium sp.
3.2.1.191 ginsenoside-transforming beta-glucosidase
-
 Microbacterium sp.
3.2.1.191 More cf. EC 3.2.1.194 Microbacterium sp.
3.2.1.194 BglG167b
-
 Microbacterium sp. Gsoil167
3.2.1.194 ginsenoside-transforming beta-glucosidase
-
 Microbacterium sp. Gsoil167
3.2.1.194 More cf. EC 3.2.1.194 Microbacterium sp. Gsoil167

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.191 45
-
 recombinant enzyme Microbacterium sp.
3.2.1.194 45
-
 recombinant enzyme Microbacterium sp. Gsoil167

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.191 30 45 at 30°C and 37°C, the recombinant enzyme has 75.0% and 89.8% of the maximal activity at 45°C Microbacterium sp.
3.2.1.194 30 45 at 30°C and 37°C, the recombinant enzyme has 75.0% and 89.8% of the maximal activity at 45°C Microbacterium sp. Gsoil167

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.191 5 30 purified recombinant enzyme, 2 h, over 90% activity remaining Microbacterium sp.
3.2.1.191 40
-
 purified recombinant enzyme, 2 h, over 80% activity remaining Microbacterium sp.
3.2.1.191 45
-
 purified recombinant enzyme, 2 h, no activity remaining, inactivation above 45°C Microbacterium sp.
3.2.1.194 5 30 purified recombinant enzyme, 2 h, over 90% activity remaining Microbacterium sp. Gsoil167
3.2.1.194 40
-
 purified recombinant enzyme, 2 h, over 80% activity remaining Microbacterium sp. Gsoil167
3.2.1.194 45
-
 purified recombinant enzyme, 2 h, no activity remaining, inactivation above 45°C Microbacterium sp. Gsoil167

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.191 7
-
 recombinant enzyme Microbacterium sp.
3.2.1.194 7
-
 recombinant enzyme Microbacterium sp. Gsoil167

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.191 5.5 9 over 50% of maximal activity within this range, recombinant enzyme Microbacterium sp.
3.2.1.194 5.5 9 over 50% of maximal activity within this range, recombinant enzyme Microbacterium sp. Gsoil167

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.191 5
-
 purified recombinant enzyme, 1.2% activity remaining Microbacterium sp.
3.2.1.191 6 9 purified recombinant enzyme, over 80% activity remaining Microbacterium sp.
3.2.1.191 8.5 9 purified recombinant enzyme, completely stable at Microbacterium sp.
3.2.1.191 10
-
 purified recombinant enzyme, 40% activity remaining Microbacterium sp.
3.2.1.194 5
-
 purified recombinant enzyme, 1.2% activity remaining Microbacterium sp. Gsoil167
3.2.1.194 6 9 purified recombinant enzyme, over 80% activity remaining Microbacterium sp. Gsoil167
3.2.1.194 8.5 9 purified recombinant enzyme, completely stable at Microbacterium sp. Gsoil167
3.2.1.194 10
-
 purified recombinant enzyme, 40% activity remaining Microbacterium sp. Gsoil167

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.191 Microbacterium sp. sequence calculation
-
 4.56
3.2.1.194 Microbacterium sp. Gsoil167 sequence calculation
-
 4.56

General Information

EC Number General Information Comment Organism
3.2.1.191 evolution the enzyme shows homology with glycosyl hydrolases from family 3, GH3 Microbacterium sp.
3.2.1.191 metabolism transformation pathways of ginsenosides by enzyme BglG167b, overview Microbacterium sp.
3.2.1.191 additional information enhanced production of gypenoside LXXV using a ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property. Anti-cancer effects of GypLXXV, Rg3(S), GypXVII and Rb1 on cell viability of HeLa cells, B16 cells, and MDA-MB23 cells Microbacterium sp.
3.2.1.194 evolution the enzyme shows homology with glycosyl hydrolases from family 3, GH3 Microbacterium sp. Gsoil167
3.2.1.194 metabolism transformation pathways of ginsenosides by enzyme BglG167b, overview Microbacterium sp. Gsoil167
3.2.1.194 additional information enhanced production of gypenoside LXXV using a ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property. Anti-cancer effects of GypLXXV, Rg3(S), GypXVII and Rb1 on cell viability of HeLa cells, B16 cells, and MDA-MB23 cells Microbacterium sp. Gsoil167