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Literature summary extracted from

  • Nishioka, K.; Rice, J.C.; Sarma, K.; Erdjument-Bromage, H.; Werner, J.; Wang, Y.; Chuikov, S.; Valenzuela, P.; Tempst, P.; Steward, R.; Lis, J.T.; Allis, C.D.; Reinberg, D.
    PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin (2002), Mol. Cell, 9, 1201-1213 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.361 expressed in Escherichia coli BL21(DE3)pLysS cells Drosophila melanogaster
2.1.1.361 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.361 R265G inactive Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.361 Ca2+ 5 mM used in assay conditions Drosophila melanogaster
2.1.1.361 Ca2+ 5 mM used in assay conditions Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.361 70000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Homo sapiens
-
 S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.361 Drosophila melanogaster
-
-
-
2.1.1.361 Homo sapiens Q9NQR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.361 DEAE-cellulose column chromatography, Superose 6 column chromatography, Mono S column chromatography, and heparin agarose column chromatography Drosophila melanogaster
2.1.1.361 DEAE-cellulose column chromatography, Superose 6 column chromatography, Mono S column chromatography, and heparin agarose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.361 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20
-
 Homo sapiens S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.361 homodimer 2 * 40000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.361 H4-K20-specific HMT
-
 Drosophila melanogaster
2.1.1.361 H4-K20-specific HMT
-
 Homo sapiens
2.1.1.361 histone H4 lysine 20 methyltransferase
-
 Drosophila melanogaster
2.1.1.361 histone H4 lysine 20 methyltransferase
-
 Homo sapiens
2.1.1.361 Pr-Set7
-
 Drosophila melanogaster
2.1.1.361 Pr-Set7
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.361 metabolism enzyme-mediated methylation of histone H4 lysine 20 maintains silent chromatin, in part, by precluding neighboring acetylation on the histone H4 tail Homo sapiens
2.1.1.361 physiological function methylation of histone H4 lysine 20 by the enzyme is essential for development and viability in Drosophila Drosophila melanogaster