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Literature summary extracted from

  • Reddi, R.; Singarapu, K.K.; Pal, D.; Addlagatta, A.
    The unique functional role of the C-HS hydrogen bond in the substrate specificity and enzyme catalysis of type 1 methionine aminopeptidase (2016), Mol. Biosyst., 12, 2408-2416 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.18 presence of a unique C-H to S hydrogen bond between the enzyme and its N-terminal-methionine polypeptide substrate, which allows specific interaction between apparent apolar interfaces, imposing a strict substrate recognition specificity and efficient catalysis Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.18 C105L inactive Mycobacterium tuberculosis
3.4.11.18 C105S 10fold decrease in kcat/Km value compared to wild-type Mycobacterium tuberculosis
3.4.11.18 C105T 4old decrease in kcat/Km value compared to wild-type Mycobacterium tuberculosis
3.4.11.18 C105V 6fold decrease in kcat/Km value compared to wild-type Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 0.94
-
 L-Met 4-nitroanilide mutant C105V, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 0.98
-
 L-Met 4-nitroanilide wild-type, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 2.47
-
 L-Met 4-nitroanilide mutant C105T, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 2.77
-
 L-Met 4-nitroanilide mutant C105S, pH 7.5, 30°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Mycobacterium tuberculosis P9WK19
-
-
3.4.11.18 Mycobacterium tuberculosis ATCC 25618 P9WK19
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 L-Met 4-nitroanilide + H2O
-
 Mycobacterium tuberculosis L-Met + 4-nitroaniline
-
 ?
3.4.11.18 L-Met 4-nitroanilide + H2O
-
 Mycobacterium tuberculosis ATCC 25618 L-Met + 4-nitroaniline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.18 additional information
-
 3.3 degrees C increase in melting temperature for methionine bound protein compared to its norleucine homolog Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 1.59
-
 L-Met 4-nitroanilide mutant C105V, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 2.36
-
 L-Met 4-nitroanilide mutant C105S, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 6.3
-
 L-Met 4-nitroanilide mutant C105T, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 10.58
-
 L-Met 4-nitroanilide wild-type, pH 7.5, 30°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.18 0.85
-
 L-Met 4-nitroanilide mutant C105S, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 1.69
-
 L-Met 4-nitroanilide mutant C105V, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 2.54
-
 L-Met 4-nitroanilide mutant C105T, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 10.79
-
 L-Met 4-nitroanilide wild-type, pH 7.5, 30°C Mycobacterium tuberculosis