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Literature summary extracted from

  • Maggioli, G.; Rinaldi, G.; Giaudrone, I.; Berasain, P.; Tort, J.F.; Brindley, P.J.; Carmona, C.
    Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni (2018), Mol. Biochem. Parasitol., 219, 17-23 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.1 DTT slight activation Schistosoma mansoni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 gene lap1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Schistosoma mansoni
3.4.11.1 gene lap2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Schistosoma mansoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline inhibits SmLAP2 Schistosoma mansoni
3.4.11.1 bestatin a specific inhibitor of aminopeptidase; a specific inhibitor of aminopeptidase, complete inhibition Schistosoma mansoni
3.4.11.1 EDTA inhibits SmLAP2, complete inhibition Schistosoma mansoni
3.4.11.1 PMSF
-
 Schistosoma mansoni
3.4.11.1 trans-epoxy-succinyl-L-leucylamido(4-guanidino)butane E64 Schistosoma mansoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ activates Schistosoma mansoni
3.4.11.1 Mn2+ activates Schistosoma mansoni
3.4.11.1 Zn2+ required Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Schistosoma mansoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Schistosoma mansoni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 adult
-
 Schistosoma mansoni
-
3.4.11.1 egg
-
 Schistosoma mansoni
-
3.4.11.1 epithelium
-
 Schistosoma mansoni
-
3.4.11.1 gut lumen Schistosoma mansoni
-
3.4.11.1 intestine
-
 Schistosoma mansoni
-
3.4.11.1 intestine presence of SmLAP1 in the lumen of the gut and in the subtegument Schistosoma mansoni
-
3.4.11.1 miracidium
-
 Schistosoma mansoni
-
3.4.11.1 additional information SmLAP1 and SmLAP2 expression in the egg and adult developmental stages of Schistosoma mansoni, from livers collected from mice 7 weeks post-infection, and in intestinal epithelia, vitelline cells and sub-tegumental regions of the parasite. Schistosoma mansoni LAPs show strong expression in the mammalian stages, i.e. schistosomules, male and female adults worms. Isozyme SmLAP2 shows higher expression than SmLAP1 in miracidia and sporocysts. Although both genes are expressed throughout the cycle, SmLAP2 is highly expressed in the molluscan infective free-living developmental stage (miracidium) and intrasnail stages. SmLAP1 is predominantly localized to gastrodermal cells lining the caecum, where digestion of hemoglobin from ingested host blood takes place Schistosoma mansoni
-
3.4.11.1 sporocyst
-
 Schistosoma mansoni
-
3.4.11.1 subcutaneous tissue
-
 Schistosoma mansoni
-
3.4.11.1 vitelline cell
-
 Schistosoma mansoni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Arg 7-amido-4-methylcoumarin + H2O moderate activity Schistosoma mansoni L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Leu 7-amido-4-methylcoumarin + H2O best substrate Schistosoma mansoni L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Leu 7-amido-4-methylcoumarin + H2O fluorogenic substrate assay Schistosoma mansoni L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Met 7-amido-4-methylcoumarin + H2O moderate activity Schistosoma mansoni L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Phe 7-amido-4-methylcoumarin + H2O low activity Schistosoma mansoni L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Thr 7-amido-4-methylcoumarin + H2O low activity Schistosoma mansoni L-Thr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Tyr 7-amido-4-methylcoumarin + H2O lower activity Schistosoma mansoni L-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 additional information substrate specificity of SmLAP2, fluorogenic substrate assay, overview. No or poor activity with L-Pro 7-amido-4-methylcoumarin, L-Gly 7-amido-4-methylcoumarin, and L-Ser 7-amido-4-methylcoumarin Schistosoma mansoni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 56700, about, sequence calculation, x * 60000, recombinant His-tagged SmLAP1, SDS-PAGE Schistosoma mansoni
3.4.11.1 ? x * 59700, about, sequence calculation, x * 63000, recombinant His-tagged SmLAP2, SDS-PAGE Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 SmLAP1
-
 Schistosoma mansoni
3.4.11.1 SmLAP2
-
 Schistosoma mansoni
3.4.11.1 Smp_03000
-
 Schistosoma mansoni
3.4.11.1 Smp_083870
-
 Schistosoma mansoni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Schistosoma mansoni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
-
 Schistosoma mansoni

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.1 Schistosoma mansoni sequence calculation
-
 6.3
3.4.11.1 Schistosoma mansoni sequence calculation
-
 7.58

General Information

EC Number General Information Comment Organism
3.4.11.1 evolution the leucine aminopeptidases (LAPs) are members of the M17 family of Zn metalloproteases Schistosoma mansoni
3.4.11.1 physiological function isozyme SmLAP1 is involved in digestion Schistosoma mansoni