Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
    A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis (2013), MicrobiologyOpen, 2, 471-481 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.74 expressed in Escherichia coli BL21(DE3) cells Pantoea ananatis
1.14.11.74 expression in Escherichia coli Pantoea ananatis
1.14.11.75 expression in Escherichia coli Pantoea ananatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.74 Fe2+ required Pantoea ananatis
1.14.11.75 Fe2+ required Pantoea ananatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis
-
 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis AJ13355
-
 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.74 Pantoea ananatis
-
-
-
1.14.11.74 Pantoea ananatis AJ13355
-
-
-
1.14.11.75 Pantoea ananatis
-
-
-
1.14.11.75 Pantoea ananatis AJ13355
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.74 Superdex 200 HR 10/30A column Pantoea ananatis

Storage Stability

EC Number Storage Stability Organism
1.14.11.74 -70°C Pantoea ananatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis 31-hydroxy-L-isoleucine + succinate + CO2
-
 ?
1.14.11.74 L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 31-hydroxy-L-isoleucine + succinate + CO2
-
 ?
1.14.11.74 L-methionine + 2-oxoglutarate + O2 low activity Pantoea ananatis L-methionine sulfoxide + succinate + CO2
-
 ?
1.14.11.74 L-methionine + 2-oxoglutarate + O2 low activity Pantoea ananatis AJ13355 L-methionine sulfoxide + succinate + CO2
-
 ?
1.14.11.74 L-valine + 2-oxoglutarate + O2 low activity Pantoea ananatis 4-hydroxy-L-valine + succinate + CO2
-
 ?
1.14.11.74 L-valine + 2-oxoglutarate + O2 low activity Pantoea ananatis AJ13355 4-hydroxy-L-valine + succinate + CO2
-
 ?
1.14.11.75 31-hydroxy-L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis (4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?
1.14.11.75 31-hydroxy-L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 (4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.74 HilA
-
 Pantoea ananatis
1.14.11.75 HilB
-
 Pantoea ananatis

General Information

EC Number General Information Comment Organism
1.14.11.74 physiological function enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine Pantoea ananatis
1.14.11.75 physiological function enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine Pantoea ananatis