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Literature summary extracted from

  • Eugster, P.J.; Salamin, K.; Grouzmann, E.; Monod, M.
    Production and characterization of two major Aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin (2015), Microbiology, 161, 2277-2288 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.26 Collagen + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.26 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.26 glycoprotein 7 glycosylation sites Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.26 Ni-NTA agarose column chromatography Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.26 culture condition:soy meal protein-grown cell
-
 Aspergillus oryzae
-
3.4.21.26 culture condition:wheat gliadin-grown cell
-
 Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 Collagen + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.21.26 Gliadin + H2O digestion of the gliadin peptide in short peptides with both enzymes S28A and S28B, occur from its N terminus Aspergillus oryzae ?
-
 ?
3.4.21.26 L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 L-Ala-L-Pro-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Pro-L-Pro + 4-nitroaniline
-
 ?
3.4.21.26 LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.21.26 additional information no activity with L-Ala-L-Pro-4-nitroanilide and L-Ala-L-Pro-L-Phe-4-nitroanilide Aspergillus oryzae ?
-
 ?
3.4.21.26 succinyl-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae succinyl-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.26 ? x * 55528, isoform S28A, calculated from amino acid sequence Aspergillus oryzae
3.4.21.26 ? x * 60000-100000, isoform S28B, SDS-PAGE Aspergillus oryzae
3.4.21.26 ? x * 60000-70000, isoform S28A, SDS-PAGE Aspergillus oryzae
3.4.21.26 ? x * 60242, isoform S28B, calculated from amino acid sequence Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.21.26 prolyl endopeptidase
-
 Aspergillus oryzae
3.4.21.26 S28A
-
 Aspergillus oryzae
3.4.21.26 S28B
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.26 4
-
 isoform S28A Aspergillus oryzae
3.4.21.26 4.5
-
 isoform S28B Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.26 2 6 isoform S28A Aspergillus oryzae
3.4.21.26 2 7 isoform S28B Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.26 Aspergillus oryzae isoform S28A, calculated from amino acid sequence
-
 4.6
3.4.21.26 Aspergillus oryzae isoform S28B, calculated from amino acid sequence
-
 5.4