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Literature summary extracted from

  • Hernandez, V.M.; Girard, L.; Hernandez-Lucas, I.; Vazquez, A.; Ortiz-Ortiz, C.; Diaz, R.; Dunn, M.F.
    Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021 (2015), Microbiology, 161, 1671-1682 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.16 expression in Escherichia coli K-12. Because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.35 L-ornithine
-
 Sinorhizobium meliloti
3.5.1.16 arginine
-
 Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.35 2.1
-
 N2-Acetyl-L-ornithine pH 8.0, 30°C Sinorhizobium meliloti
2.3.1.35 8.2
-
 L-glutamate pH 8.0, 30°C Sinorhizobium meliloti
3.5.1.16 4
-
 N2-Acetyl-L-ornithine pH 8.0, 30°C Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.16 CoCl2 metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2 Sinorhizobium meliloti
3.5.1.16 MnCl2 metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2. MnCl2 (0.2 mM) added to the reaction in place of CoCl2 restores activity to about 40 % of that seen with CoCl2 Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.35 88000
-
 gel filtration Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Sinorhizobium meliloti
-
 L-ornithine + N-acetyl-L-glutamate
-
 ?
3.5.1.16 N2-acetyl-L-ornithine + H2O Sinorhizobium meliloti
-
 acetate + L-ornithine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Sinorhizobium meliloti Q92MJ1
-
-
3.5.1.16 Sinorhizobium meliloti Q92Y75
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.35 proteolytic modification autoproteolysis occurs between A194/T195, which results in generation of alpha and beta polypeptides Sinorhizobium meliloti

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.35
-
 Sinorhizobium meliloti
3.5.1.16 because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Sinorhizobium meliloti L-ornithine + N-acetyl-L-glutamate
-
 ?
3.5.1.16 N2-acetyl-L-ornithine + H2O
-
 Sinorhizobium meliloti acetate + L-ornithine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.35 tetramer 1 * 23000 (alpha) + 2 * 25000 (beta) Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
2.3.1.35 argJ
-
 Sinorhizobium meliloti
3.5.1.16 ArgE
-
 Sinorhizobium meliloti
3.5.1.16 N-acetylornithine deacetylase
-
 Sinorhizobium meliloti
3.5.1.16 NAO deacetylase
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.35 30
-
 assay at Sinorhizobium meliloti
3.5.1.16 30
-
 assay at Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.35 8
-
 assay at Sinorhizobium meliloti
3.5.1.16 8
-
 assay at Sinorhizobium meliloti

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.35 2.4
-
 pH 8.0, 30°C Sinorhizobium meliloti L-ornithine

General Information

EC Number General Information Comment Organism
2.3.1.35 malfunction inactivation of argJ does not cause arginine auxotrophy or marked growth defects Sinorhizobium meliloti
3.5.1.16 malfunction inactivation of argE gene does not cause arginine auxotrophy or marked growth defects Sinorhizobium meliloti