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Literature summary extracted from

  • Negi, V.S.; Borthakur, D.
    Heterologous expression and characterization of mimosinase from Leucaena leucocephala (2016), Methods Mol. Biol., 1405, 59-77 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.61 generation of a synthetic gene, cloning method overview, subcloning in Escherichia coli strain JM109, sequence comparison with wild-type gene, recombinant enzyme expression of the synthetic gene syn-mimosinase ORF in Escherichia coli strain BL21(DE3) pLysS Leucaena leucocephala

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.61 L-Phe competitive inhibition Leucaena leucocephala
3.5.1.61 L-Trp competitive inhibition Leucaena leucocephala
3.5.1.61 L-Tyr competitive inhibition Leucaena leucocephala

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.61 chloroplast the enzyme sequence contains a chloroplast transit peptide Leucaena leucocephala 9507
-
3.5.1.61 additional information the enzyme sequence contains a 43 amino acid signal peptide Leucaena leucocephala
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.61 mimosine + H2O Leucaena leucocephala
-
 3-hydroxy-4-(1H)-pyridone + pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.61 Leucaena leucocephala A8CEI3 collected from University of Hawaii research station, Waimanalo, Honolulu
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.61 seed mature Leucaena leucocephala
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.61 mimosine + H2O
-
 Leucaena leucocephala 3-hydroxy-4-(1H)-pyridone + pyruvate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.61 37
-
-
 Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.61 8
-
-
 Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.61 pyridoxal 5'-phosphate dependent on Leucaena leucocephala

General Information

EC Number General Information Comment Organism
3.5.1.61 physiological function mimosinase is an important enzyme especially in the context of metabolic engineering of plant secondary metabolite as it catalyzes the degradation of mimosine, which is a toxic secondary metabolite found in all Leucaena and Mimosa species Leucaena leucocephala