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Literature summary extracted from

  • Mora-Montes, H.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Flores-Carreon, A.
    Heterologous expression and biochemical characterization of an alpha1,2-mannosidase encoded by the Candida albicans MNS1 gene (2008), Mem. Inst. Oswaldo Cruz, 103, 724-730 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.113 expressed in Escherichia coli BL21 Star (DE3) cells Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin
-
 Candida albicans
3.2.1.113 swainsonine
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.113 0.07
-
 4-methylumbelliferyl alpha-D-mannopyranoside at pH 6.0 and 37°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 endoplasmic reticulum
-
 Candida albicans 5783
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Candida albicans Q8J0Q0
-
-
3.2.1.113 Candida albicans ATCC 26555 Q8J0Q0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 0.0007
-
 crude enzyme, at pH 6.0 and 37°C Candida albicans
3.2.1.113 0.1081
-
 after 154fold purification, at pH 6.0 and 37°C Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.113 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Candida albicans ATCC 26555 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + H2O
-
 Candida albicans Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + H2O
-
 Candida albicans ATCC 26555 Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?
3.2.1.113 additional information the enzyme fails to process Man5GlcNAc2-Asn Candida albicans ?
-
 ?
3.2.1.113 additional information the enzyme fails to process Man5GlcNAc2-Asn Candida albicans ATCC 26555 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 ? x * 65000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.113 alpha1,2-mannosidase
-
 Candida albicans
3.2.1.113 MNS1
-
 Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 6
-
-
 Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.113 0.19
-
 at pH 6.0 and 37°C Candida albicans 1-deoxymannojirimycin
3.2.1.113 0.62
-
 at pH 6.0 and 37°C Candida albicans swainsonine

General Information

EC Number General Information Comment Organism
3.2.1.113 metabolism the enzyme is involved in the processing of N-linked mannans Candida albicans