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Literature summary extracted from

  • Couttas, T.A.; Lim, X.Y.; Don, A.S.
    A three-step assay for ceramide synthase activity using a fluorescent substrate and HPLC (2015), Lipids, 50, 101-109 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.297 gene CERS2, recombinant expression in HEK-293 cells Homo sapiens
2.3.1.299 gene CERS1, recombinant expression in HEK-293 cells Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.297 Mg2+ activates Homo sapiens
2.3.1.299 Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.297 dihydrosphingosine + lignoceroyl-CoA Homo sapiens
-
 N-lignoceroyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + nervonoyl-CoA Homo sapiens
-
 N-nervonoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 dihydrosphingosine + palmitoyl-CoA Homo sapiens low activity N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 dihydrosphingosine + stearoyl-CoA Homo sapiens highly preferred substrate N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 additional information Homo sapiens clear preference for the C18:0 NBD-dihydroceramide synthesis is observed with extracts of human frontal cortex grey matter ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.297 Homo sapiens Q96G23
-
-
2.3.1.299 Homo sapiens P27545
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.297 brain
-
 Homo sapiens
-
2.3.1.297 U-87MG cell
-
 Homo sapiens
-
2.3.1.299 brain
-
 Homo sapiens
-
2.3.1.299 neuron CERS1 is highly expressed in neurons Homo sapiens
-
2.3.1.299 U-87MG cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.297 dihydrosphingosine + lignoceroyl-CoA
-
 Homo sapiens N-lignoceroyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + lignoceroyl-CoA low activity Homo sapiens N-lignoceroyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + nervonoyl-CoA
-
 Homo sapiens N-nervonoyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + nervonoyl-CoA highly preferred substrate Homo sapiens N-nervonoyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + palmitoyl-CoA low activity Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.297 dihydrosphingosine + stearoyl-CoA low activity Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.297 additional information CERS2 preferentially transfers very long chain fatty acids (C22:0-C26:0) to dihydrosphingosine. Improvement of a fluorescent assay, using HPLC using C16:0, C18:0 and C24:1 fatty acids and commercially available sphinganine-NBD, i.e. 7-nitro-2-1,3-benzoxadiazole-labelled dihydrosphingosine or (2S,3R)-2-amino-18((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)octadecane-1-3-diol, as substrates, very accurate and sensitive method for quantification of CERS activity, method evaluation Homo sapiens ?
-
 ?
2.3.1.299 dihydrosphingosine + palmitoyl-CoA low activity Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 dihydrosphingosine + stearoyl-CoA highly preferred substrate Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 additional information clear preference for the C18:0 NBD-dihydroceramide synthesis is observed with extracts of human frontal cortex grey matter Homo sapiens ?
-
 ?
2.3.1.299 additional information CERS1-expressing HEK293 cell extracts show a clear preference for utilisation of the C18:0 fatty acid substrate when presented with equimolar concentrations of C16:0, C18:0, C24:0, and C24:1 fatty acid substrates. CERS1 preferentially catalyses the addition of 18-carbon (C18:0) fatty acids to dihydrosphingosine. No activity with lignoceroyl-CA, and poor activity with nervonoyl-CoA. Improvement of a fluorescent assay, using HPLC using C16:0, C18:0, and C24:1 fatty acids and commercially available sphinganine-NBD, i.e. 7-nitro-2-1,3-benzoxadiazole-labelled dihydrosphingosine or (2S,3R)-2-amino-18((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)octadecane-1-3-diol, as substrates, very accurate and sensitive method for quantification of CERS activity, method evaluation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.297 ceramide synthase 2
-
 Homo sapiens
2.3.1.297 CerS2
-
 Homo sapiens
2.3.1.299 ceramide synthase 1
-
 Homo sapiens
2.3.1.299 CerS1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.297 37
-
 assay at Homo sapiens
2.3.1.299 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.297 7.4
-
 assay at Homo sapiens
2.3.1.299 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.297 physiological function very long chain ceramides synthesized by CERS2 are essential constituents of myelin. CERS2 demonstrates a preference for the monounsaturated C24:1 fatty acid substrate compared to the saturated C24:0 substrate, potentially explaining why myelin is enriched in ceramides containing the monounsaturated form of very long chain fatty acids Homo sapiens
2.3.1.299 physiological function C18 ceramide synthesized by CERS1 is implicated in apoptosis and lethal autophagy Homo sapiens