Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shimeno, H.; Soeda, S.; Sakamoto, M.; Kouchi, T.; Kowakame, T.; Kihara, T.
    Partial purification and characterization of sphingosine N-acyltransferase (ceramide synthase) from bovine liver mitochondrion-rich fraction (1998), Lipids, 33, 601-605 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
-
 Bos taurus
2.3.1.24 Triton X-100
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.24 additional information
-
 additional information kinetics and substrate specificity, overview Bos taurus
2.3.1.24 0.141
-
 palmitoyl-CoA pH 7.5, 37°C Bos taurus
2.3.1.24 0.144
-
 sphinganine pH 7.5, 37°C Bos taurus
2.3.1.24 0.146
-
 stearoyl-CoA pH 7.5, 37°C Bos taurus
2.3.1.24 0.171
-
 sphingosine pH 7.5, 37°C Bos taurus
2.3.1.24 0.18
-
 oleoyl-CoA pH 7.5, 37°C Bos taurus
2.3.1.24 0.299
-
 behenoyl-CoA pH 7.5, 37°C Bos taurus
2.3.1.24 0.358
-
 lauroyl-CoA pH 7.5, 37°C Bos taurus
2.3.1.24 0.522
-
 hexanoyl-CoA pH 7.5, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 mitochondrial membrane
-
 Bos taurus 31966
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.24 240000 260000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Bos taurus A0A3Q1MVP2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.24 native enzyme 98.7fold from liver mitochondrionrich fraction by solubilization with n-octyl beta-D-thioglucoside, ion exchange chromatography, sphingosine affinity chromatography, and gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.24 0.0148
-
 purified enzyme, pH 7.5, 37°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 additional information substrate specificity of the purified enzyme is determined with various acyl (C6-C22)-CoA and sphingosine or with stearoyl-CoA and sphinganine as substrates. CoA esters having regular chain (C16-C18) are good substrates. Very long chain (C22) or short chain (C6-C12) show low activity Bos taurus ?
-
 ?
2.3.1.24 sphinganine + stearoyl-CoA
-
 Bos taurus N-stearoylsphinganine + CoA
-
 ?
2.3.1.24 sphingosine + behenoyl-CoA low activity Bos taurus N-behenoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + hexanoyl-CoA very low activity Bos taurus N-hexanoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + lauroyl-CoA very low activity Bos taurus N-lauroylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + oleoyl-CoA
-
 Bos taurus N-oleoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + palmitoyl-CoA
-
 Bos taurus N-palmitoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + stearoyl-CoA
-
 Bos taurus N-stearoylsphingosine + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.24 tetramer 4 * 62000-72000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase
-
 Bos taurus
2.3.1.24 ceramide synthase 5
-
 Bos taurus
2.3.1.24 sphingosine N-acyltransferase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.24 7 8 over 70% of maximal activity within this range Bos taurus