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Literature summary extracted from

  • Krishnapura, P.; Belur, P.
    Partial purification and characterization of L-asparaginase from an endophytic Talaromyces pinophilus isolated from the rhizomes of Curcuma amada (2016), J. Mol. Catal. B, 124, 83-91 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 dithiothreitol 0.5 mM, 12% increase in activity Talaromyces pinophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Ca2+ 10 mM, 90% residual activity Talaromyces pinophilus
3.5.1.1 Cu2+ 10 mM, 92% residual activity Talaromyces pinophilus
3.5.1.1 SDS 10 mM, 30% residual activity Talaromyces pinophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 6.4
-
 L-asparagine pH 8.0, 37°C Talaromyces pinophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 101000
-
 PAGE Talaromyces pinophilus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Talaromyces pinophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 partial Talaromyces pinophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 23.7
-
 substrate L-glutamine, pH 8.0, 37°C Talaromyces pinophilus
3.5.1.1 145
-
 substrate L-asparagine, pH 8.0, 37°C Talaromyces pinophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Talaromyces pinophilus L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Talaromyces pinophilus L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer 1 * 62000 + 1 * 39000, SDS-PAGE Talaromyces pinophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 28
-
-
 Talaromyces pinophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 26 37
-
 Talaromyces pinophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 286.3
-
 L-asparagine pH 8.0, 37°C Talaromyces pinophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Talaromyces pinophilus