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Literature summary extracted from

  • STressler, T.; Eisele, T.; Kranz, B.; Fischer, L.
    PepX from Lactobacillus helveticus Automated multi-step purification and determination of kinetic parameters with original tripeptide substrates (2014), J. Mol. Catal. B, 108, 103-110 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.11 0.12
-
 Tyr-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.14
-
 Ala-Pro-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.24
-
 Arg-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.54
-
 Ser-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 1.53
-
 Ala-Pro-4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.11 Lactobacillus helveticus S4TJ23
-
-
3.4.14.11 Lactobacillus helveticus ATCC 12046 S4TJ23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.11
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.11 22.2
-
 pH 6.5, 37°C Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.11 Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus ATCC 12046 Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Ala-Pro-Arg + H2O
-
 Lactobacillus helveticus Ala-Pro + Arg
-
 ?
3.4.14.11 Ala-Pro-Arg + H2O
-
 Lactobacillus helveticus ATCC 12046 Ala-Pro + Arg
-
 ?
3.4.14.11 Arg-Pro-Ala + H2O
-
 Lactobacillus helveticus Arg-Pro + Ala
-
 ?
3.4.14.11 Arg-Pro-Ala + H2O
-
 Lactobacillus helveticus ATCC 12046 Arg-Pro + Ala
-
 ?
3.4.14.11 Ser-Pro-Ala + H2O
-
 Lactobacillus helveticus Ser-Pro + Ala
-
 ?
3.4.14.11 Ser-Pro-Ala + H2O
-
 Lactobacillus helveticus ATCC 12046 Ser-Pro + Ala
-
 ?
3.4.14.11 Tyr-Pro-Ala + H2O
-
 Lactobacillus helveticus Tyr-Pro + Ala
-
 ?
3.4.14.11 Tyr-Pro-Ala + H2O
-
 Lactobacillus helveticus ATCC 12046 Tyr-Pro + Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.11 ? x * 90000, SDS-PAGE Lactobacillus helveticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.11 57.2
-
 Ala-Pro-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 128
-
 Ala-Pro-4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 133
-
 Arg-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 145
-
 Tyr-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 233
-
 Ser-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.11 83.4
-
 Ala-Pro-4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 409
-
 Ala-Pro-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 431
-
 Ser-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 552
-
 Arg-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 1210
-
 Tyr-Pro-Ala pH 6.5, 37°C Lactobacillus helveticus