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Literature summary extracted from

  • Zhong, F.L.; Ma, R.; Jiang, M.; Dong, W.W.; Jiang, J.; Wu, S.; Li, D.; Quan, L.H.
    Cloning and characterization of ginsenoside-hydrolyzing beta-glucosidase from Lactobacillus brevis that Ttransforms ginsenosides Rb1 and F2 into ginsenoside Rd and compound K (2016), J. Microbiol. Biotechnol., 26, 1661-1667 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.193 synthesis in pharmaceutical and commercial industries, this recombinant Bgy2 can be suitable for producting ginsenoside Rd and compound K Levilactobacillus brevis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.193 gene bgy2, recombinant expression in Escherichia coli strain BL21(DE3) Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.193 ginsenoside F2 + H2O Levilactobacillus brevis
-
 compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside F2 + H2O Levilactobacillus brevis KB290
-
 compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O Levilactobacillus brevis
-
 ginsenoside Rd + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O Levilactobacillus brevis KB290
-
 ginsenoside Rd + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.193 Levilactobacillus brevis M5AXT3 isolated from a fermented food in Korea called kimchi
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3.2.1.193 Levilactobacillus brevis KB290 M5AXT3 isolated from a fermented food in Korea called kimchi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.193 2-nitrophenyl-beta-D-glucopyranoside + H2O 93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Levilactobacillus brevis 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.193 2-nitrophenyl-beta-D-glucopyranoside + H2O 93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Levilactobacillus brevis KB290 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.193 4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Levilactobacillus brevis 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.193 4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Levilactobacillus brevis KB290 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.193 ginsenoside F2 + H2O
-
 Levilactobacillus brevis compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside F2 + H2O
-
 Levilactobacillus brevis KB290 compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O
-
 Levilactobacillus brevis ginsenoside Rd + D-glucose
-
 ?
3.2.1.193 ginsenoside Rb1 + H2O
-
 Levilactobacillus brevis KB290 ginsenoside Rd + D-glucose
-
 ?
3.2.1.193 additional information beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry Levilactobacillus brevis ?
-
 ?
3.2.1.193 additional information beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry Levilactobacillus brevis KB290 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.193 bgy2
-
 Levilactobacillus brevis
3.2.1.193 ginsenoside-hydrolyzing beta-glucosidase
-
 Levilactobacillus brevis
3.2.1.193 LVISKB_0241 locus name Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.193 30
-
 recombinant enzyme Levilactobacillus brevis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.193 20 70 about 95% of maximal activity at 20°C, about 30°C at 50°C, and 10% at 70°C Levilactobacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.193 20 30 purified recombinnat enzyme, pH 7.0, completely stable at Levilactobacillus brevis
3.2.1.193 40
-
 purified recombinnat enzyme, pH 7.0, about 85% activity remaining Levilactobacillus brevis
3.2.1.193 50
-
 purified recombinnat enzyme, pH 7.0, complete inactivation Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.193 7
-
 recombinant enzyme Levilactobacillus brevis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.193 3.5 8.5 activity range, profile overview, recombinant enzyme Levilactobacillus brevis

General Information

EC Number General Information Comment Organism
3.2.1.193 evolution the enzyme is a member of glycosyl hydrolase family 3, GH3 Levilactobacillus brevis