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Literature summary extracted from

  • Li, J.; Wei, H.; Zhou, M.M.
    Structure-guided design of a methyl donor cofactor that controls a viral histone H3 lysine 27 methyltransferase activity (2011), J. Med. Chem., 54, 7734-7738 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.356 expressed in Escherichia coli BL21(DE3) cells Paramecium bursaria Chlorella virus 1

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.356 L116A the mutant shows strongly increased activity compared to the wild type enzyme Paramecium bursaria Chlorella virus 1
2.1.1.356 Y109G the mutant shows strongly decreased activity compared to the wild type enzyme Paramecium bursaria Chlorella virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.356 0.06
-
 S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.062
-
 S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.072
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.086
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.086
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.126
-
 S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.128
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.137
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.173
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.917
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 2.8
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.356 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Paramecium bursaria Chlorella virus 1 overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 Paramecium bursaria Chlorella virus 1
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 Paramecium bursaria Chlorella virus 1
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 Paramecium bursaria Chlorella virus 1
-
 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.356 Paramecium bursaria Chlorella virus 1 O41094
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.356 heparin column chromatography and Superdex 75 gel filtration Paramecium bursaria Chlorella virus 1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.356 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 ?
-
 ?
2.1.1.356 (+/-)-2'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 ?
-
 ?
2.1.1.356 (+/-)-3'-benzyl-S-adenosyl-L-methionine + [histone H3]-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 ?
-
 ?
2.1.1.356 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 overall reaction Paramecium bursaria Chlorella virus 1 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
-
 ?
2.1.1.356 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27
-
 Paramecium bursaria Chlorella virus 1 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.356 EZH2
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 histone H3 lysine 27 methyltransferase
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 Polycomb repressive complex 2
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 PRC2
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 SET
-
 Paramecium bursaria Chlorella virus 1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.356 0.000008
-
 S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.000008
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.00004
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.002
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.002
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.002
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0025
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.006
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0217
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.065
-
 S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.123
-
 S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.356 0.000003
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.000043
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.000128
-
 S-adenosyl-L-methionine mutant enzyme Y109G, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0156
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0183
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0252
-
 (+/-)-2'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.027
-
 (+/-)-3'-benzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.0357
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.3
-
 (+/-)-2',3'-dibenzyl-S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 0.516
-
 S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1
2.1.1.356 2.056
-
 S-adenosyl-L-methionine mutant enzyme L116A, at pH 8.0 and 23°C Paramecium bursaria Chlorella virus 1