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Literature summary extracted from

  • Liu, L.; Qu, M.; Liu, T.; Chen, Q.; Guo, X.; Yang, J.; Yang, Q.
    Biochemical characterization of three midgut chitin deacetylases of the Lepidopteran insect Bombyx mori (2019), J. Insect Physiol., 113, 42-48 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41 CDA6 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori
3.5.1.41 CDA7 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori
3.5.1.41 CDA8 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.41 colloidal chitin + H2O Bombyx mori
-
 ?
-
 ?
3.5.1.41 peritrophic matrix chitin + H2O Bombyx mori
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Bombyx mori H9JW43
-
-
3.5.1.41 Bombyx mori H9JW44
-
-
3.5.1.41 Bombyx mori H9JW45
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant His-tagged isozyme BmCDA6 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori
3.5.1.41 recombinant His-tagged isozyme BmCDA7 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori
3.5.1.41 recombinant His-tagged isozyme BmCDA8 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 midgut
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 additional information
-
 enzymatic activity as micromol acetate/min/micromol of protein Bombyx mori
3.5.1.41 0.061
-
 substrate peritrophic matrix chitin, isozyme BmCDA8, pH 7.4, 30°C Bombyx mori
3.5.1.41 0.078
-
 substrate colloidal chitin, isozyme BmCDA6, pH 7.4, 30°C Bombyx mori
3.5.1.41 0.207
-
 substrate colloidal chitin, isozyme BmCDA8, pH 7.4, 30°C Bombyx mori
3.5.1.41 0.284
-
 substrate peritrophic matrix chitin, isozyme BmCDA7, pH 7.4, 30°C Bombyx mori
3.5.1.41 0.393
-
 substrate colloidal chitin, isozyme BmCDA7, pH 7.4, 30°C Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 colloidal chitin + H2O
-
 Bombyx mori ?
-
 ?
3.5.1.41 additional information no activity with peritrophic matrix chitin by isozyme BmCDA6 Bombyx mori ?
-
 ?
3.5.1.41 peritrophic matrix chitin + H2O
-
 Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 41400, about, isozyme BmCDA7 , sequence calculation Bombyx mori
3.5.1.41 ? x * 41500, about, isozyme BmCDA8 , sequence calculation Bombyx mori
3.5.1.41 ? x * 41700, about, isozyme BmCDA6, sequence calculation Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 BmCDA6
-
 Bombyx mori
3.5.1.41 BmCDA7
-
 Bombyx mori
3.5.1.41 BmCDA8
-
 Bombyx mori
3.5.1.41 CDA
-
 Bombyx mori
3.5.1.41 midgut chitin deacetylase
-
 Bombyx mori
3.5.1.41 NodB homology domain-containing protein UniProt Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 30
-
 assay at Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 7.4
-
 assay at Bombyx mori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.41 Bombyx mori sequence calculation
-
 5.13
3.5.1.41 Bombyx mori sequence calculation
-
 5.21
3.5.1.41 Bombyx mori sequence calculation
-
 5.71