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Literature summary extracted from

  • Kaur, T.; Kaur, A.; Grewal, R.K.
    Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions (2015), J. Food Sci. Technol., 52, 5954-5960 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.33 Ca2+ noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 Ananas comosus
3.4.22.33 Cu2+ competitive inhibition at pH 3.5 with 0.6 mM Cu2+, which changes to an uncompetitive inhibition at pH 4.5 and pH 7.5 Ananas comosus
3.4.22.33 Hg2+ uncompetitive inhibition at pH 3.5 and pH 4.5 with 0.6 mM Hg2+ Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.33 additional information
-
 additional information Michaelis-Menten kinetics Ananas comosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.33 Ca2+ noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 Ananas comosus
3.4.22.33 additional information modulation of fruit bromelain by divalent ions is pH dependent Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.33 Ananas comosus O23791
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.33 native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.33 fruit
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.33 2512.44
-
 purified native enzyme, pH and temperature not specified in the publication Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.33 Gelatin + H2O
-
 Ananas comosus ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.33 additional information
-
 additional information inhibition kinetics, detailed overview Ananas comosus