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Literature summary extracted from

  • Yilmaz, B.; Baltaci, M.O.; Sisecioglu, M.; Adiguzel, A.
    Thermotolerant alkaline protease enzyme from Bacillus licheniformis A10 purification, characterization, effects of surfactants and organic solvents (2016), J. Enzyme Inhib. Med. Chem., 31, 1241-1247 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.63 2-mercaptoethanol 94% activation at 1 mM, 70% at 5 mM Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 acetone loss of 64-74% activity at 15-50% v/v after 24 h, loss of 27-70% activity after 1 h Bacillus licheniformis
3.4.21.63 Ag+ about 19% inhibition at 1-10 mM Bacillus licheniformis
3.4.21.63 butanol loss of 40-68% activity at 15-50% v/v after 24 h, loss of 40-47% activity after 1 h Bacillus licheniformis
3.4.21.63 chloroform loss of 45-88.5% activity at 15-50% v/v after 24 h, loss of 31-21% activity after 1 h Bacillus licheniformis
3.4.21.63 Co2+ 8% inhibition at 10 mM Bacillus licheniformis
3.4.21.63 DMSO loss of 41-53% activity at 15-50% v/v after 24 h, loss of 21-48% activity after 1 h Bacillus licheniformis
3.4.21.63 DTNB 52-57% inhibition at 1-5 mM Bacillus licheniformis
3.4.21.63 EDTA complete inhibition at 5 mM, 50% at 1 mM Bacillus licheniformis
3.4.21.63 ethanol loss of 55-78% activity at 15-50% v/v after 24 h, loss of 8-55% activity after 1 h Bacillus licheniformis
3.4.21.63 Fe2+ 45% inhibition at 10 mM, 33.5% at 5 mM Bacillus licheniformis
3.4.21.63 formaldehyde loss of 27-13% activity at 15-50% v/v after 24 h, loss of 46-47% activity after 1 h Bacillus licheniformis
3.4.21.63 H2O2 73-74% inhibition at 1-5 mM Bacillus licheniformis
3.4.21.63 Isopropanol loss of 39-45% activity at 15-50% v/v after 24 h, loss of about 50% activity after 1 h Bacillus licheniformis
3.4.21.63 methanol loss of 75-100% activity at 15-50% v/v after 24 h, loss of 44-93% activity after 1 h Bacillus licheniformis
3.4.21.63 Ni2+ 32% inhibition at 10 mM, 28% at 5 mM Bacillus licheniformis
3.4.21.63 PMSF complete inhibition at 5 mM, 97% at 1 mM Bacillus licheniformis
3.4.21.63 SDS complete inhibition at 5 mM, 96% at 1 mM Bacillus licheniformis
3.4.21.63 Triton X-100 45% inhibition at 5 mM, 39% at 1 mM Bacillus licheniformis
3.4.21.63 Tween 20 66% inhibition at 5 mM, 39% at 1 mM Bacillus licheniformis
3.4.21.63 Tween 80 72% inhibition at 1-5 mM Bacillus licheniformis
3.4.21.63 Urea 71% inhibition at 1 mM, 95.5% at 8 mM Bacillus licheniformis
3.4.21.63 Zn2+ 40% inhibition at 10 mM, 38% at 1-5 mM Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.63 additional information
-
 additional information Km and Vmax values of the protease enzyme purified from strain A10 are 0.033 mg/ml and 8.17 mmol/ml/min, respectively, with casein as substrate Bacillus licheniformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.63 extracellular
-
 Bacillus licheniformis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 K+ activates 15% at 10 mM Bacillus licheniformis
3.4.21.63 Mg2+ activates 5% at 10 mM Bacillus licheniformis
3.4.21.63 Mn2+ activates 14.5% at 10 mM Bacillus licheniformis
3.4.21.63 additional information no effect on activity by Ca2+ Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Bacillus licheniformis
-
 originally isolated from Erzurum Ilica hot springs
-
3.4.21.63 Bacillus licheniformis A10
-
 originally isolated from Erzurum Ilica hot springs
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63 native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 azocasein + H2O 97% activity compared to casein Bacillus licheniformis ?
-
 ?
3.4.21.63 azocasein + H2O 97% activity compared to casein Bacillus licheniformis A10 ?
-
 ?
3.4.21.63 Bovine serum albumin + H2O 18% activity compared to casein Bacillus licheniformis ?
-
 ?
3.4.21.63 Bovine serum albumin + H2O 18% activity compared to casein Bacillus licheniformis A10 ?
-
 ?
3.4.21.63 casein + H2O the enzyme has a high affinity for the casein, best substrate Bacillus licheniformis ?
-
 ?
3.4.21.63 casein + H2O the enzyme has a high affinity for the casein, best substrate Bacillus licheniformis A10 ?
-
 ?
3.4.21.63 Gelatin + H2O 28% activity compared to casein Bacillus licheniformis ?
-
 ?
3.4.21.63 Gelatin + H2O 28% activity compared to casein Bacillus licheniformis A10 ?
-
 ?
3.4.21.63 Hemoglobin + H2O 60% activity compared to casein Bacillus licheniformis ?
-
 ?
3.4.21.63 Hemoglobin + H2O 60% activity compared to casein Bacillus licheniformis A10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.63 ? x * 40550, extracellular enzyme, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.63 Alkaline protease
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.63 60 70
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.63 20 70 over 70% of maximal activity within this range, profile overview Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 20 80 purified native extracellular enzyme, over 50% activity remaining after 15-30 min. After 60-min incubation, the enzyme preserves 70.74%, 54.22%, 94.82%, and 98.47% of its activity at 40°C, 50°C, 60°C, and 70°C, respectively Bacillus licheniformis
3.4.21.63 60 70 purified native extracellular enzyme, 1 h, 90% activity remaining Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.63 1.392
-
 casein pH 8.0, 37°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 9
-
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.63 6 11 activity range, 70% of maximal activity at pH 6.0, 60% at pH 11.0, profile overview Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 6
-
 purified native extracellular enzyme, 40% of activity remaining Bacillus licheniformis
3.4.21.63 7.5 10.5 purified native extracellular enzyme, over 60% activity remianing within this range Bacillus licheniformis
3.4.21.63 11
-
 purified native extracellular enzyme, 30% of activity remaining Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.4.21.63 additional information the enzyme is a serine protease Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.63 1.392
-
 casein pH 8.0, 37°C Bacillus licheniformis