Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Macomber, L.; Minkara, M.S.; Hausinger, R.P.; Merz, K.M.
    Reduction of urease activity by interaction with the flap covering the active site (2015), J. Chem. Inf. Model., 55, 354-361 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.5 expression in Escherichia coli BL21(DE3) Klebsiella aerogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.5 C319A mutation increases the urease resistance to both epigallocatechin and quercetin Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 epigallocatechin strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance Klebsiella aerogenes
3.5.1.5 additional information caftaric acid, desmosine, kanamycin, and labetalol, each examined at concentrations up to 0.6 mM, show little to no inhibitory activity Klebsiella aerogenes
3.5.1.5 quercetin strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.5 urea + H2O Klebsiella aerogenes
-
 CO2 + 2 NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Klebsiella aerogenes P18314
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Klebsiella aerogenes CO2 + 2 NH3
-
 ?