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Literature summary extracted from

  • Wangler, N.J.; Jayaraman, S.; Zhu, R.; Mechref, Y.; Abbruscato, T.J.; Bickel, U.; Karamyan, V.T.
    Preparation and preliminary characterization of recombinant neurolysin for in vivo studies (2016), J. Biotechnol., 234, 105-115 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.16 additional information catalytic activity of recombinant Nln increases over time upon incubation in fresh mouse blood. The recombinant protein easily reaches the systemiccirculation remaining both catalytically active and physically intact Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.16 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.24.16 up to two cycles of freezing and thawing of the purified rNln do not significantly affect the purified recombinant enzyme's specific activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.16 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.16 Ca2+ required Rattus norvegicus
3.4.24.16 Zn2+ zinc metalloproteinase Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.16 Rattus norvegicus P42676
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.16 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration to homogeneity, large scale production of rNln Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.24.16 a gradual decrease of the specific activity is observed in preparations kept longer than 6 months at -80°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.16 angiotensin I + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.16 bradykinin + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.16 neurotensin + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.16 ? x * 75000, recombinant His6-tagged enzyme, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.16 Nln
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.16 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.16 7.2
-
 assay at Rattus norvegicus