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Literature summary extracted from

  • Shin, K.; Lee, H.; Oh, D.
    Substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides and its application in the production of ginsenosides Rg3, Rg2, and Rh1 from ginseng root extract (2015), J. Biosci. Bioeng., 119, 497-504 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.192 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Gordonia terrae
3.2.1.194 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Gordonia terrae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.192 additional information
-
additional information Michaelis-Menten kinetics Gordonia terrae
3.2.1.192 2.46
-
ginsenoside Rb1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 2.74
-
ginsenoside Rd recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 14.66
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 additional information
-
additional information Michaelis-Menten kinetics Gordonia terrae
3.2.1.194 6.61
-
ginsenoside Rg1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 6.74
-
ginsenoside Re recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 14.66
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.192 156000
-
recombinant His-tagged enzyme, gel filtration Gordonia terrae
3.2.1.194 156000
-
recombinant His-tagged enzyme, gel filtration Gordonia terrae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.192 ginsenoside Rb1 + H2O Gordonia terrae
-
ginsenoside Rd + D-glucose
-
?
3.2.1.192 ginsenoside Rd + H2O Gordonia terrae
-
ginsenoside Rg3 + D-glucose
-
?
3.2.1.194 ginsenoside F1 + H2O Gordonia terrae
-
protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside F1 + H2O Gordonia terrae DSM 43249
-
protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside F1 + H2O Gordonia terrae NBRC 100016
-
protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside Re + H2O Gordonia terrae
-
ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Re + H2O Gordonia terrae DSM 43249
-
ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Re + H2O Gordonia terrae NBRC 100016
-
ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Rg1 + H2O Gordonia terrae
-
ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 ginsenoside Rg1 + H2O Gordonia terrae DSM 43249
-
ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 ginsenoside Rg1 + H2O Gordonia terrae NBRC 100016
-
ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 notoginsenoside R1 + H2O Gordonia terrae
-
notoginsenoside R2 + D-glucose
-
?
3.2.1.194 notoginsenoside R1 + H2O Gordonia terrae DSM 43249
-
notoginsenoside R2 + D-glucose
-
?
3.2.1.194 notoginsenoside R1 + H2O Gordonia terrae NBRC 100016
-
notoginsenoside R2 + D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.192 Gordonia terrae
-
-
-
3.2.1.192 Gordonia terrae DSM 43249
-
-
-
3.2.1.192 Gordonia terrae NBRC 100016
-
-
-
3.2.1.194 Gordonia terrae
-
-
-
3.2.1.194 Gordonia terrae DSM 43249
-
-
-
3.2.1.194 Gordonia terrae NBRC 100016
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.192 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Gordonia terrae
3.2.1.194 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Gordonia terrae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.192 additional information
-
effectivity of ginsenoside production, overview Gordonia terrae
3.2.1.192 16.4
-
purified recombinant enzyme, substrate ginsenoside Rd, pH 6.5, 30°C Gordonia terrae
3.2.1.192 250
-
purified recombinant enzyme, substrate ginsenoside Rb1, pH 6.5, 30°C Gordonia terrae
3.2.1.194 additional information
-
effectivity of ginsenoside production, overview Gordonia terrae
3.2.1.194 1
-
purified recombinant enzyme, substrate ginsenoside Rh1, pH 6.5, 30°C Gordonia terrae
3.2.1.194 11
-
purified recombinant enzyme, substrate notoginsenoside R1, pH 6.5, 30°C Gordonia terrae
3.2.1.194 11.5
-
purified recombinant enzyme, substrate ginsenoside Rg1, pH 6.5, 30°C Gordonia terrae
3.2.1.194 15.9
-
purified recombinant enzyme, substrate ginsenoside Re, pH 6.5, 30°C Gordonia terrae
3.2.1.194 132
-
purified recombinant enzyme, substrate ginsenoside F1, pH 6.5, 30°C Gordonia terrae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.192 2-nitrophenyl-beta-D-glucopyranoside + H2O
-
Gordonia terrae 2-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.192 4-nitrophenyl-beta-D-glucopyranoside + H2O
-
Gordonia terrae 4-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.192 ginsenoside Rb1 + H2O
-
Gordonia terrae ginsenoside Rd + D-glucose
-
?
3.2.1.192 ginsenoside Rd + H2O
-
Gordonia terrae ginsenoside Rg3 + D-glucose
-
?
3.2.1.192 additional information substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside Gordonia terrae ?
-
?
3.2.1.194 2-nitrophenyl-beta-D-glucopyranoside + H2O
-
Gordonia terrae 2-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.194 4-nitrophenyl-beta-D-glucopyranoside + H2O
-
Gordonia terrae 4-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.194 ginsenoside F1 + H2O
-
Gordonia terrae protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside F1 + H2O
-
Gordonia terrae DSM 43249 protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside F1 + H2O
-
Gordonia terrae NBRC 100016 protopanaxatriol + D-glucose
-
?
3.2.1.194 ginsenoside Re + H2O
-
Gordonia terrae ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Re + H2O
-
Gordonia terrae DSM 43249 ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Re + H2O
-
Gordonia terrae NBRC 100016 ginsenoside Rg2 + L-rhamnose
-
?
3.2.1.194 ginsenoside Rg1 + H2O
-
Gordonia terrae ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 ginsenoside Rg1 + H2O
-
Gordonia terrae DSM 43249 ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 ginsenoside Rg1 + H2O
-
Gordonia terrae NBRC 100016 ginsenoside Rh1 + D-glucose
-
?
3.2.1.194 additional information substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside Gordonia terrae ?
-
?
3.2.1.194 additional information substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside Gordonia terrae DSM 43249 ?
-
?
3.2.1.194 additional information substrate specificity of beta-glucosidase from Gordonia terrae for ginsenosides, overview. The purified recombinant enzyme specifically hydrolyzes the glucopyranosides at the C-20 position in protopanaxadiol (PPD)-type ginsenosides and hydrolyzes the glucopyranoside at the C-6 or C-20 position in protopanaxatriol (PPT)-type ginsenosides. The enzyme is a beta-glucosidase with xylopyranoside-hydrolyzing activity that is active with 4-nitrophenyl-beta-D-xylopyranoside and 2-nitrophenyl-beta-D-xylopyranoside. The specific activity for PPD-type ginsenosides as substrates is in the order gypenoside XVII > gypenoside LXXV > Rb1 > F2 > compound K > Rd, and the products are F2, compound K, Rd, Rh2, APPD, and Rg3, respectively. The specific activity for PPT-type ginsenosides as substrates follows the order F1 > Re > Rg1 > notoginsenoside R1 > Rh1 and the products of which are APPT, Rg2, Rh1, notoginsenoside R2, and APPT, respectively. No activity with ginsenosides Rb2, Rc, Compound Mc, Compound Mc-1, Compound O, and Compound Y, as well as with notoginsenoside R2 and ginsenoside Rg2. No activity is found for 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranose, 4-nitrophenyl-alpha-L-arabinopyranose, or 4-nitrophenyl-alpha-L-rhamnopyranoside Gordonia terrae NBRC 100016 ?
-
?
3.2.1.194 notoginsenoside R1 + H2O
-
Gordonia terrae notoginsenoside R2 + D-glucose
-
?
3.2.1.194 notoginsenoside R1 + H2O
-
Gordonia terrae DSM 43249 notoginsenoside R2 + D-glucose
-
?
3.2.1.194 notoginsenoside R1 + H2O
-
Gordonia terrae NBRC 100016 notoginsenoside R2 + D-glucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.192 More see also EC 3.2.1.194 and EC 3.2.1.191 Gordonia terrae
3.2.1.194 More see also EC 3.2.1.192 and EC 3.2.1.191 Gordonia terrae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.192 30
-
recombinant enzyme Gordonia terrae
3.2.1.194 30
-
recombinant enzyme Gordonia terrae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.192 15 50 activity range, inactive above 50°C, recombinant enzyme Gordonia terrae
3.2.1.194 15 50 activity range, inactive above 50°C, recombinant enzyme Gordonia terrae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.192 6.06
-
ginsenoside Rd recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 82.87
-
ginsenoside Rb1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 6292
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 5.75
-
ginsenoside Rg1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 7.3
-
ginsenoside Re recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 6292
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.192 6.5
-
recombinant enzyme Gordonia terrae
3.2.1.194 6.5
-
recombinant enzyme Gordonia terrae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.192 5.5 7.5 about 60% of maximal activity pH 5.5 and pH 7.5 Gordonia terrae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.192 2.21
-
ginsenoside Rd recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 33.69
-
ginsenoside Rb1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.192 1057.7
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 0.87
-
ginsenoside Rg1 recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 1.08
-
ginsenoside Re recombinant enzyme, pH 6.5, 30°C Gordonia terrae
3.2.1.194 1057.7
-
4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, pH 6.5, 30°C Gordonia terrae