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Literature summary extracted from

  • Reddy, V.S.; Singh, A.K.; Rajasekharan, R.
    The Saccharomyces cerevisiae PHM8 gene encodes a soluble magnesium-dependent lysophosphatidic acid phosphatase (2008), J. Biol. Chem., 283, 8846-8854 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.106 expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.106 D58E inactive Saccharomyces cerevisiae
3.1.3.106 D58N inactive Saccharomyces cerevisiae
3.1.3.106 D60E 33.5% of wild-type activity Saccharomyces cerevisiae
3.1.3.106 D60N inactive Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.106 EDTA complete inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.106 0.034
-
 oleoyl-sn-glycerol-3-phosphate pH 6.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.106 soluble
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.106 Co2+ 76% of the activity with Mg2+ Saccharomyces cerevisiae
3.1.3.106 Mg2+ required, maximum activity at 2 mM Saccharomyces cerevisiae
3.1.3.106 Mn2+ 48% of the activity with Mg2+ Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.106 Saccharomyces cerevisiae
-
-
-
3.1.3.106 Saccharomyces cerevisiae BY4741
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.106 oleoyl-sn-glycerol-3-phosphate + H2O
-
 Saccharomyces cerevisiae oleoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 oleoyl-sn-glycerol-3-phosphate + H2O
-
 Saccharomyces cerevisiae BY4741 oleoyl-sn-glycerol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.106 Phm8
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.106 6.5
-
-
 Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
3.1.3.106 Saccharomyces cerevisiae expression is upregulated in response to phosphate-starving conditions up

General Information

EC Number General Information Comment Organism
3.1.3.106 physiological function a gene deletion mutant shows decreased lysophosphatidic acid-hydrolyzing activity under phosphate-limiting conditions. Overexpression of PHM8 in yeast results in an increase in the lysophosphatidic acid phosphatase activity in vivo Saccharomyces cerevisiae