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Literature summary extracted from

  • Spassieva, S.; Seo, J.G.; Jiang, J.C.; Bielawski, J.; Alvarez-Vasquez, F.; Jazwinski, S.M.; Hannun, Y.A.; Obeid, L.M.
    Necessary role for the Lag1p motif in (dihydro)ceramide synthase activity (2006), J. Biol. Chem., 281, 33931-33938 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.299 transient recombinant expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HeLa cells, overexpression of wild-type LASS1 increases cellular levels of C18 and C18:1 ceramides by 10fold Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.299 D210N site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 D213N site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 H182D site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 H183D site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 H220D site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 K220L site-directed mutagenesis, mutation in the Lag1p motif, the mutant enzyme is able to increase cellular C18 and C18:1 ceramide levels to a similar or slightly lesser extent than wild-type LASS1, the mutation does not affect the fatty acid chain-length specificity Mus musculus
2.3.1.299 L189E site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 L216E site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 L254E site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 L254M site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus
2.3.1.299 additional information the some mutants shows altered specificity with substrates of different chain lengths, overview Mus musculus
2.3.1.299 S193A site-directed mutagenesis, mutation in the Lag1p motif, the mutant enzyme is able to increase cellular C18 and C18:1 ceramide levels to a similar or slightly lesser extent than wild-type LASS1, mutation does not affect the fatty acid chain-length specificity Mus musculus
2.3.1.299 S231A site-directed mutagenesis, mutation in the Lag1p motif, the mutant shows highly reduced activity, but the mutation does not affect its fatty acid chain-length specificity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.299 fumonisin B1 an in vitro inhibitor of (dihydro)-ceramide synthase, inhibits wild-type and mutant enzymes in vivo in HeLa cells Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.299 microsome
-
 Mus musculus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.299 Mus musculus P27545
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.299 additional information C18:0 fatty acid CoA, but not cis-C18:1 fatty acid CoAs, are substrates for LASS1 Mus musculus ?
-
 ?
2.3.1.299 sphingosine + stearoyl-CoA
-
 Mus musculus N-stearoylsphingosine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.299 (dihydro)-ceramide synthase
-
 Mus musculus
2.3.1.299 ceramide synthase 1
-
 Mus musculus
2.3.1.299 CerS1
-
 Mus musculus
2.3.1.299 LASS1
-
 Mus musculus
2.3.1.299 longevity assurance homologue 1 of yeast Lag1
-
 Mus musculus
2.3.1.299 UOG1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.299 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.299 7.4
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.299 evolution Lag1 (longevity assurance gene 1) homologues, a family of transmembrane proteins found in all eukaryotes, is necessary for (dihydro)ceramide synthesis. All Lag1 homologues contain a highly conserved stretch of 52 amino acids known as the Lag1p motif, sequence comparisons, analysis of functional significance of the conserved Lag1p motif for (dihydro)ceramide synthesis, overview. The Lag1p motif is essential for the enzyme activity of all Lag1 homologues Mus musculus