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Literature summary extracted from

  • Koshkin, A.; Nunn, C.M.; Djordjevic, S.; Ortiz de Montellano, P.R.
    The mechanism of Mycobacterium tuberculosis alkylhydroperoxidase AhpD as defined by mutagenesis, crystallography, and kinetics (2003), J. Biol. Chem., 278, 29502-29508 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.28 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.28 hanging drop vapor diffusion method, crystal structures of the H137F and H132Q mutants Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.28 C130S expressed in yield comparable to that of the wild-type protein. Mutation decreases activity to less than 5% of the wild-type enzyme Mycobacterium tuberculosis
1.11.1.28 C133S expressed in yield comparable to that of the wild-type protein. Mutation completely suppresses the activity Mycobacterium tuberculosis
1.11.1.28 E118F expressed at 20% of the wild-type expression level. Mutation decreases catalytic activity Mycobacterium tuberculosis
1.11.1.28 E118Q expressed at 20% of the wild-type expression level. Mutation modestly loweres the catalytic activity Mycobacterium tuberculosis
1.11.1.28 H132F expressed in yield comparable to that of the wild-type protein. Mutation decreases the catalytic activity Mycobacterium tuberculosis
1.11.1.28 H132Q mutant tends to unfold and degrade relatively easily. Mutation decreases the catalytic activity. The H137Q mutation decreases the activity more severely than the H132Q mutation Mycobacterium tuberculosis
1.11.1.28 H137F expressed in yield comparable to that of the wild-type protein. Mutation decreases the catalytic activity Mycobacterium tuberculosis
1.11.1.28 H137Q expressed at 20% of the wild-type expression level. Mutation decreases the catalytic activity. The H137Q mutation decreases the activity more severely than the H132Q mutation Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.28 Mycobacterium tuberculosis P9WQB5
-
-
1.11.1.28 Mycobacterium tuberculosis ATCC 25618 P9WQB5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.28
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.28 AhpD
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.28 7.2
-
 wild-type enzyme Mycobacterium tuberculosis
1.11.1.28 7.5
-
 mutant enzymes H137F and H132F Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.11.1.28 physiological function the enzyme provides antioxidant protection Mycobacterium tuberculosis