Literature summary extracted from
Lobsanov, Y.D.; Vallee, F.; Imberty, A.; Yoshida, T.; Yip, P.; Herscovics, A.; Howell, P.L.
Structure of Penicillium citrinum alpha-1,2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and golgi class I enzymes (2002), J. Biol. Chem., 277, 5620-5630 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.113 |
expressed in Aspergillus oryzae strain MS2 |
Penicillium citrinum |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.113 |
modeling of structure at 2.2 A resolution |
Penicillium citrinum |
3.2.1.113 |
native enzyme and in complexes with the inhibitors 1-deoxymannojirimycin and kifunensine, hanging drop vapor diffusion method, using 17-22% (w/v) polyethylene glycol 6000, 50 mM KH2PO4, pH 4.6 |
Penicillium citrinum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.113 |
1-deoxymannojirimycin |
modeling of binding to enzyme |
Penicillium citrinum |
|
3.2.1.113 |
kifunensine |
modeling of binding to enzyme |
Penicillium citrinum |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.2.1.113 |
Ca2+ |
required |
Penicillium citrinum |
|
3.2.1.113 |
Ca2+ |
inhibitors kifunensine and 1-deoxymannojirimycin bind to the essential Ca2+ ion |
Penicillium citrinum |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.2.1.113 |
Man9GlcNAc2 + H2O |
Penicillium citrinum |
- |
Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.113 |
Penicillium citrinum |
- |
- |
- |
3.2.1.113 |
Penicillium citrinum |
P31723 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.113 |
ammonium sulfate precipitation and CM-Toyopearl 650M column chromatography |
Penicillium citrinum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.113 |
Man9GlcNAc2 + H2O |
- |
Penicillium citrinum |
Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.113 |
homodimer |
x-ray crystallography |
Penicillium citrinum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.113 |
alpha1,2-mannosidase |
- |
Penicillium citrinum |
3.2.1.113 |
msdC |
- |
Penicillium citrinum |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.2.1.113 |
physiological function |
comparison of the molecular structure of the Penicillium citrinum Golgi enzyme, and yeast and human endoplasmic reticulum enzymes. There is a greater degree of freedom to bind the oligosaccharide in the active site of the fungal enzyme than in the yeast and human ER alpha1,2-mannosidases |
Penicillium citrinum |