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Literature summary extracted from

  • Lobsanov, Y.D.; Vallee, F.; Imberty, A.; Yoshida, T.; Yip, P.; Herscovics, A.; Howell, P.L.
    Structure of Penicillium citrinum alpha-1,2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and golgi class I enzymes (2002), J. Biol. Chem., 277, 5620-5630 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.113 expressed in Aspergillus oryzae strain MS2 Penicillium citrinum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.113 modeling of structure at 2.2 A resolution Penicillium citrinum
3.2.1.113 native enzyme and in complexes with the inhibitors 1-deoxymannojirimycin and kifunensine, hanging drop vapor diffusion method, using 17-22% (w/v) polyethylene glycol 6000, 50 mM KH2PO4, pH 4.6 Penicillium citrinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin modeling of binding to enzyme Penicillium citrinum
3.2.1.113 kifunensine modeling of binding to enzyme Penicillium citrinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Ca2+ required Penicillium citrinum
3.2.1.113 Ca2+ inhibitors kifunensine and 1-deoxymannojirimycin bind to the essential Ca2+ ion Penicillium citrinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.113 Man9GlcNAc2 + H2O Penicillium citrinum
-
 Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Penicillium citrinum
-
-
-
3.2.1.113 Penicillium citrinum P31723
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 ammonium sulfate precipitation and CM-Toyopearl 650M column chromatography Penicillium citrinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Man9GlcNAc2 + H2O
-
 Penicillium citrinum Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 homodimer x-ray crystallography Penicillium citrinum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.113 alpha1,2-mannosidase
-
 Penicillium citrinum
3.2.1.113 msdC
-
 Penicillium citrinum

General Information

EC Number General Information Comment Organism
3.2.1.113 physiological function comparison of the molecular structure of the Penicillium citrinum Golgi enzyme, and yeast and human endoplasmic reticulum enzymes. There is a greater degree of freedom to bind the oligosaccharide in the active site of the fungal enzyme than in the yeast and human ER alpha1,2-mannosidases Penicillium citrinum