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Literature summary extracted from

  • Taniguchi, H.; Sungwallek, S.; Chotchuang, P.; Okano, K.; Honda, K.
    A key enzyme of the NAD+ salvage pathway in Thermus thermophilus characterization of nicotinamidase and the impact of its gene deletion at high temperatures (2017), J. Bacteriol., 199, e00359-17 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.017
-
nicotinamide pH and temperature not specified in the publication Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Thermus thermophilus key enzyme of the NAD+ salvage pathway nicotinate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Thermus thermophilus Q5SLG5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
Thermus thermophilus nicotinate + NH3
-
?
3.5.1.19 nicotinamide + H2O key enzyme of the NAD+ salvage pathway Thermus thermophilus nicotinate + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 TTHA0328
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Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.19 65
-
-
Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.19 50 80 50°C: about 75% of maximal activity, 80°C: about 60% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.19 60
-
1 h, 5% loss of activity Thermus thermophilus
3.5.1.19 80
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1 h, 80% loss of activity Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.19 50
-
nicotinamide pH and temperature not specified in the publication Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 9
-
-
Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.19 6 9.5 above 50% of the maximum activity is confirmed at a broad range of pHs from 6.0 to 9.5 Thermus thermophilus

General Information

EC Number General Information Comment Organism
3.5.1.19 malfunction deletion of this gene abolishes nicotinamide deamination activity in crude extracts of Thermus thermophilus and disrupts the NAD+ salvage pathway in Thermus thermophilus. Disruption of the salvage pathway leads to the severe growth retardation at a higher temperature (80°C), owing to the drastic decrease in the intracellular concentrations of NAD+ and NADH Thermus thermophilus
3.5.1.19 physiological function key enzyme of the NAD+ salvage pathway Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.19 3000
-
nicotinamide pH and temperature not specified in the publication Thermus thermophilus