EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 0.017 | - |
nicotinamide | pH and temperature not specified in the publication | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.19 | nicotinamide + H2O | Thermus thermophilus | key enzyme of the NAD+ salvage pathway | nicotinate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.19 | Thermus thermophilus | Q5SLG5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.19 | nicotinamide + H2O | - |
Thermus thermophilus | nicotinate + NH3 | - |
? | |
3.5.1.19 | nicotinamide + H2O | key enzyme of the NAD+ salvage pathway | Thermus thermophilus | nicotinate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.19 | TTHA0328 | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 65 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 50 | 80 | 50°C: about 75% of maximal activity, 80°C: about 60% of maximal activity | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 60 | - |
1 h, 5% loss of activity | Thermus thermophilus |
3.5.1.19 | 80 | - |
1 h, 80% loss of activity | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 50 | - |
nicotinamide | pH and temperature not specified in the publication | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 9 | - |
- |
Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 6 | 9.5 | above 50% of the maximum activity is confirmed at a broad range of pHs from 6.0 to 9.5 | Thermus thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.19 | malfunction | deletion of this gene abolishes nicotinamide deamination activity in crude extracts of Thermus thermophilus and disrupts the NAD+ salvage pathway in Thermus thermophilus. Disruption of the salvage pathway leads to the severe growth retardation at a higher temperature (80°C), owing to the drastic decrease in the intracellular concentrations of NAD+ and NADH | Thermus thermophilus |
3.5.1.19 | physiological function | key enzyme of the NAD+ salvage pathway | Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 3000 | - |
nicotinamide | pH and temperature not specified in the publication | Thermus thermophilus |