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Literature summary extracted from

  • Fabre, E.; Bozonnet, S.; Arcache, A.; Willemot, R.M.; Vignon, M.; Monsan, P.; Remaud-Simeon, M.
    Role of the two catalytic domains of DSR-E dextransucrase and their involvement in the formation of highly alpha-1,2 branched dextran (2005), J. Bacteriol., 187, 296-303 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.373 expression in Escherichia coli Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.373 additional information expression of truncated forms of enzyme containing either catalytic domain CD1 or CD2. Enzymes devoid of CD2 synthesize products containing only alpha-1,6 linkages. Enzymes devoid of CD1 modified alpha-1,6 linear oligosaccharides and dextran acceptors through the formation of alpha-1,2 linkages Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.373 Leuconostoc mesenteroides Q8G9Q2
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.373 additional information recombinant protein DSR-E synthesizes both alpha-1,6 and alpha-1,2 glucosidic linkages in transglucosylation reactions using sucrose as the donor and maltose as the acceptor. Each of the two catalytic domains is highly regiospecific, CD1 being specific for the synthesis of alpha-1,6 glucosidic bonds and CD2 only catalyzing the formation of alpha-1,2 linkages Leuconostoc mesenteroides ?
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?
2.4.1.373 sucrose + a (1->6)-alpha-D-glucan
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Leuconostoc mesenteroides D-fructose + a (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
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?
2.4.1.373 sucrose + alpha-1,6-dextran
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Leuconostoc mesenteroides D-fructose + alpha-1,6-dextran containing a (1->2)-alpha-D-glucose branch
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?
2.4.1.373 sucrose + maltose
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Leuconostoc mesenteroides D-fructose + ?
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?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.373 dextransucrase
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Leuconostoc mesenteroides
2.4.1.373 DsrE
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Leuconostoc mesenteroides