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Literature summary extracted from

  • Tourneux, L.; Bucurenci, N.; Saveanu, C.; Kaminski, P.A.; Bouzon, M.; Pistotnik, E.; Namane, A.; Marliere, P.; Barzu, O.; Li De La Sierra, I.; Neuhard, J.; Gilles, A.M.
    Genetic and biochemical characterization of Salmonella enterica serovar Typhi deoxyribokinase (2000), J. Bacteriol., 182, 869-873 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.229 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhi

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.229 M10N mutation to the coresponding residue of ribokinase, decreases the Vmax of deoxyribokinase by a factor of 2.5 and increases the Km for deoxyribose by a factor of 70, compared to the parent enzyme Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.229 0.1
-
 2-deoxy-D-ribose wild-type, pH 7.4, 30°C Salmonella enterica subsp. enterica serovar Typhi
2.7.1.229 2
-
 D-ribose wild-type, pH 7.4, 30°C Salmonella enterica subsp. enterica serovar Typhi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.229 ATP + 2-deoxy-D-ribose Salmonella enterica subsp. enterica serovar Typhi
-
 ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
2.7.1.229 ATP + 2-deoxy-D-ribose Salmonella enterica subsp. enterica serovar Typhi Ty2
-
 ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.229 Salmonella enterica subsp. enterica serovar Typhi
-
-
-
2.7.1.229 Salmonella enterica subsp. enterica serovar Typhi Ty2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.229 ATP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhi ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
2.7.1.229 ATP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhi Ty2 ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
2.7.1.229 ATP + D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhi ADP + D-ribose 5-phosphate
-
 ?
2.7.1.229 ATP + D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhi Ty2 ADP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.229 ? x * 33228, mass spectrometry, x * 33229, calulated from sequence Salmonella enterica subsp. enterica serovar Typhi