EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.143 | purified enzyme in complex with ADP-ribose dimer, X-ray diffraction structure determination and analysis at 1.9 A resolution | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.143 | E755N | site-directed mutagenesis, inactive mutant | Homo sapiens |
3.2.1.143 | E756N | site-directed mutagenesis, inactive mutant | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.143 | ADP-HPD | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.143 | poly(ADP-ribose) + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.143 | Homo sapiens | Q86W56 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.143 | 2'-O-(ADP-ribosyl)-adenosine 5'-phosphate + H2O | - |
Homo sapiens | ? | - |
? | |
3.2.1.143 | ADP-ribose dimer + H2O | the N-1 adenine group interacts with the amide nitrogen of the conserved Leu752 in human PARG, while the beta-phosphate forms H-bonds with the conserved Ala750, enzyme-substrate binding structure, crystal structure analysis, overview | Homo sapiens | 2 ADP-ribose | - |
? | |
3.2.1.143 | additional information | synthesis of derivatives of the ADP-ribose dimer and development of a PAR Binding assay, overview. Propargyl ADP-ribose dimer binds to E756N and E755N mutants of human PARG with KD values of 83 nM and 208 nM, respectively. Fluorescent propargyl ADP-ribose dimer does not bind proteins devoid of PAR binding activity such as bovine serum albumin even at high concentrations of protein. Wild-type PARG is unable to process the truncated substrates 2'-O-alpha-D-ribofuranosyladenosine and 2'-O-(5-O-phosphono-a-D-ribofuranosyl)adenosine 5'-phosphate) | Homo sapiens | ? | - |
? | |
3.2.1.143 | poly(ADP-ribose) + H2O | - |
Homo sapiens | ? | - |
? | |
3.2.1.143 | propargyl ADP-ribose dimer + H2O | - |
Homo sapiens | propargyl ADP-ribose + ADP-ribose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.143 | PARG | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.143 | physiological function | poly(ADP-ribosyl)ation is a common post-translational modification that mediates a wide variety of cellular processes including DNA damage repair, chromatin regulation, transcription, and apoptosis, involving interactions of PAR with poly(ADP-ribose) glycohydrolase (PARG) and other binding proteins | Homo sapiens |