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Literature summary extracted from

  • Guo, T.; Ouyang, X.; Xin, Y.; Wang, Y.; Zhang, S.; Kong, J.
    Characterization of a new cell envelope proteinase PrtP from Lactobacillus rhamnosus CGMCC11055 (2016), J. Agric. Food Chem., 64, 6985-6992 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.96 Ba2+ 1 mM, 82% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Cu2+ 1 mM, 77% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 EDTA 1 mM, 45% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 EGTA 1 mM, 52% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Fe2+ 1 mM, 82% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 K+ 1 mM, 79% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Li+ 1 mM, 89% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Mn2+ 1 mM, 78% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 p-amidinophenylmethylsulfonyl fluoride
-
 Lacticaseibacillus rhamnosus
3.4.21.96 PMSF 1 mM, 12% residual activity Lacticaseibacillus rhamnosus
3.4.21.96 Sn2+ 1 mM, 77% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Sr2+ 1 mM, 93% of initial activity Lacticaseibacillus rhamnosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.96 Ca2+ 1 mM, 106% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Co2+ 1 mM, 112% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Mg2+ 1 mM, 109% of initial activity Lacticaseibacillus rhamnosus
3.4.21.96 Zn2+ 1 mM, 108% of initial activity Lacticaseibacillus rhamnosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.96 170000
-
 above 170000 Da, PAGE Lacticaseibacillus rhamnosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.96 Lacticaseibacillus rhamnosus
-
-
-
3.4.21.96 Lacticaseibacillus rhamnosus CGMCC11055
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.96
-
 Lacticaseibacillus rhamnosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.96 alphas1-casein + H2O
-
 Lacticaseibacillus rhamnosus ?
-
 ?
3.4.21.96 alphas1-casein + H2O
-
 Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?
3.4.21.96 beta-casein + H2O preferred substrate Lacticaseibacillus rhamnosus ?
-
 ?
3.4.21.96 beta-casein + H2O preferred substrate Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?
3.4.21.96 additional information proline is the preferred residue at the cleavage site. No substrate: kappa-casein Lacticaseibacillus rhamnosus ?
-
 ?
3.4.21.96 additional information proline is the preferred residue at the cleavage site. No substrate: kappa-casein Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.96 monomer 1 * more than 170000 Da, PAGE Lacticaseibacillus rhamnosus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.96 PrtP
-
 Lacticaseibacillus rhamnosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.96 44
-
-
 Lacticaseibacillus rhamnosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.96 40
-
 30 min, pH 6.5, stable Lacticaseibacillus rhamnosus
3.4.21.96 50
-
 30 min, pH 6.5, 20% residual activity Lacticaseibacillus rhamnosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.96 6.5
-
-
 Lacticaseibacillus rhamnosus