EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.168 | additional information | rutinose-containing glycoconjugates of phenolics might have antiviral activity and by useful as therapeutics/antiviral agents | Fagopyrum tataricum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.168 | Fagopyrum tataricum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.168 | native enzyme from seeds by anion exchange chromatography and dialysis | Fagopyrum tataricum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.168 | seed | - |
Fagopyrum tataricum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.168 | additional information | catalysis of rutin hydrolysis (EC 3.2.1.168) and transglycosylation of phenol acceptors by rutinase. Preparation of rutinosides of phenolic acids (vanillic acid, sinapic acid, ferulic acid, and caffeic acid) using the reverse reaction of hydrolysis by rutinase, derived from tartary buckwheat (Fagopyrum tataricum) seeds. Rutinase is used to transfer the rutinose moiety from rutin (sugar donor) to the phenolic acids. The transglycosylation products are detected by NMR spectroscopy. Rutinosylation proceeds at the primary hydroxyl group of rutinose. In the rutinosylation of vanillic acid, sinapic acid, and ferulic acid, a hydroxyl group at the 4-position of the aromatic ring was rutinosylated. When caffeic acid, which contains two hydroxyl groups in the ortho position, is used as the acceptor, the hydroxyl group at the 3-position of the aromatic ring was conjugated to rutinose. Rutinase has high substrate specificity for rutin (quercetin-3-rutinoside) and does not degrade structural homologues such as naringin (naringenin-7-rhamnoglucoside) and quercitrin (quercetin 3-rhamnoside). Phenolic compounds containing two terminal aromatic groups, such as curcumin, nordihydroguaiaretic acid (NDGA), and resveratrol, do not form rutinosides | Fagopyrum tataricum | ? | - |
? | |
3.2.1.168 | rutin + caffeic acid | - |
Fagopyrum tataricum | quercetin + caffeic acid 3'-O-rutinoside | - |
? | |
3.2.1.168 | rutin + ferulic acid | - |
Fagopyrum tataricum | quercetin + ferulic acid 4'-O-rutinoside | - |
? | |
3.2.1.168 | rutin + sinapic acid | - |
Fagopyrum tataricum | quercetin + sinapic acid 4'-O-rutinoside | - |
? | |
3.2.1.168 | rutin + vanillic acid | - |
Fagopyrum tataricum | quercetin + vanillic acid 4'-O-rutinoside | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.168 | rutinase | - |
Fagopyrum tataricum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.168 | 40 | - |
- |
Fagopyrum tataricum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.168 | 60 | - |
the purified native enzyme is stable up to 60°C | Fagopyrum tataricum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.168 | 5 | - |
- |
Fagopyrum tataricum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.168 | 4 | 7 | the purified native enzyme is stable at pH 4.0-7.0 | Fagopyrum tataricum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.168 | additional information | determination of antiviral activity against FCV strain F9 propagated and titrated on CRFK cells | Fagopyrum tataricum |
3.2.1.168 | physiological function | phenolic compounds (phenolic acids, flavonoids, lignans, and tannins) and especially phenolic glycosides often show activity against several viruses such as rotavirus, herpes virus, and influenza virus. Glycosylation can potentiate the antiviral effect of the phenolic compound | Fagopyrum tataricum |